A common beta-sheet architecture underlies in vitro and in vivo beta2-microglobulin amyloid fibrils.

The Journal of Biological Chemistry
Thomas R JahnSheena E Radford

Abstract

Misfolding and aggregation of normally soluble proteins into amyloid fibrils and their deposition and accumulation underlies a variety of clinically significant diseases. Fibrillar aggregates with amyloid-like properties can also be generated in vitro from pure proteins and peptides, including those not known to be associated with amyloidosis. Whereas biophysical studies of amyloid-like fibrils formed in vitro have provided important insights into the molecular mechanisms of amyloid generation and the structural properties of the fibrils formed, amyloidogenic proteins are typically exposed to mild or more extreme denaturing conditions to induce rapid fibril formation in vitro. Whether the structure of the resulting assemblies is representative of their natural in vivo counterparts, thus, remains a fundamental unresolved issue. Here we show using Fourier transform infrared spectroscopy that amyloid-like fibrils formed in vitro from natively folded or unfolded beta(2)-microglobulin (the protein associated with dialysis-related amyloidosis) adopt an identical beta-sheet architecture. The same beta-strand signature is observed whether fibril formation in vitro occurs spontaneously or from seeded reactions. Comparison of these spect...Continue Reading

References

Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·G A TennentM B Pepys
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Oct 3, 1999·Methods in Enzymology·G A Tennent
Oct 3, 1999·Methods in Enzymology·S SeshadriA L Fink
Mar 15, 2000·Protein Science : a Publication of the Protein Society·S GodaK Yutani
Aug 2, 2001·Journal of Molecular Biology·J L JiménezH R Saibil
Aug 17, 2001·Journal of the American Chemical Society·J ZurdoC M Dobson
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian O'Nuallain, Ronald Wetzel
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Hidenori KatouYuji Goto
Nov 8, 2002·The Journal of Biological Chemistry·Alessia AndreolaMargaret Sunde
Jul 10, 2003·Journal of Molecular Biology·Neil M KadNeil H Thomson
Jul 16, 2003·Journal of Molecular Biology·Susan JonesSheena E Radford
Jan 14, 2004·Protein Science : a Publication of the Protein Society·Aoneng CaoLuhua Lai
May 12, 2004·Biochimica Et Biophysica Acta·Vladimir N Uversky, Anthony L Fink
Jul 9, 2004·Journal of Molecular Biology·Erica FrareAngelo Fontana
Jul 13, 2004·Biomacromolecules·Brian A VernagliaEliana D Clark
Nov 13, 2004·Protein Science : a Publication of the Protein Society·Giorgia ZandomeneghiMarcus Fändrich
Jan 25, 2005·Journal of Molecular Biology·Geoffrey W PlattSheena E Radford
Jul 19, 2005·Journal of Molecular Biology·Walraj S GosalSheena E Radford
Sep 13, 2005·Biochimica Et Biophysica Acta·Monica StoppiniVittorio Bellotti
Sep 20, 2005·Biophysical Journal·Martino CalamaiChristopher M Dobson
Nov 24, 2005·The FEBS Journal·O Sumner Makin, Louise C Serpell
Nov 24, 2005·The FEBS Journal·Thomas R Jahn, Sheena E Radford
Jan 18, 2006·Annual Review of Medicine·Mark B Pepys
Feb 24, 2006·Nature Structural & Molecular Biology·Thomas R JahnSheena E Radford
Mar 28, 2006·Current Opinion in Structural Biology·Rebecca Nelson, David Eisenberg
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Jun 15, 2006·Quarterly Reviews of Biophysics·Robert Tycko
Sep 20, 2006·Accounts of Chemical Research·Witold K SurewiczAdrian C Apetri
Oct 19, 2006·Methods in Enzymology·Kimberly L SciarrettaStephen C Meredith
Jan 26, 2007·Current Opinion in Structural Biology·Ravindra Kodali, Ronald Wetzel
Feb 10, 2007·Journal of Structural Biology·Minna GroenningFlemming S Jørgensen
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samrat MukhopadhyayAshok A Deniz

❮ Previous
Next ❯

Citations

Mar 26, 2009·Der Pathologe·S SchremlP Babilas
Apr 2, 2010·Journal of the American Chemical Society·Emeline Barbet-MassinGuido Pintacuda
Jul 29, 2010·Journal of the American Chemical Society·Galia T DebelouchinaRobert G Griffin
Jan 28, 2009·Nature Structural & Molecular Biology·Matthew F CalabreseAndrew D Miranker
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lilia MilanesiHelen R Saibil
Oct 8, 2009·The Journal of Biological Chemistry·Wei-Feng XueSheena E Radford
Oct 22, 2008·The Journal of Biological Chemistry·Nathan J CobbWitold K Surewicz
Oct 5, 2013·The Journal of Chemical Physics·Joseph FoleyMartin Muschol
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kevin W TippingSheena E Radford
May 27, 2016·European Biophysics Journal : EBJ·Dominic NarangSamrat Mukhopadhyay
Jan 25, 2011·Molecular Cell·Timo EichnerSheena E Radford
Mar 9, 2017·International Journal of Molecular Sciences·Ajda Taler-VerčičEva Žerovnik
Aug 7, 2009·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Niels H H Heegaard
May 21, 2011·The FEBS Journal·Timo Eichner, Sheena E Radford
Apr 19, 2019·The Journal of Biological Chemistry·Núria Benseny-CasesSheena E Radford
Oct 31, 2018·Nature Communications·Matthew G IadanzaSheena E Radford
Jul 8, 2020·Chemical Society Reviews·Pu Chun KeRaffaele Mezzenga
Jul 11, 2012·Rapid Communications in Mass Spectrometry : RCM·John P HodkinsonAlison E Ashcroft
Sep 22, 2018·Nature Reviews. Molecular Cell Biology·Matthew G IadanzaSheena E Radford
Nov 17, 2012·Journal of Molecular Biology·Atsushi MukaiyamaKunihiro Kuwajima
Oct 15, 2009·Journal of the American Chemical Society·Mirela MustataRuth Nussinov
Nov 17, 2010·Journal of the American Chemical Society·Galia T DebelouchinaRobert G Griffin
Aug 29, 2019·ACS Omega·Mouli KonarSwagata Dasgupta

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
light
x-ray fiber diffraction
NMR
x-ray crystallography
light microscopy
state

Software Mentioned

SigmaPlot
NMRPipe

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.