PMID: 8457544Mar 23, 1993Paper

A common mechanism for influenza virus fusion activity and inactivation

Biochemistry
J Ramalho-SantosM da C de Lima

Abstract

The fusion of influenza virus (A/PR/8/34 strain) with PC-12 cells was monitored by a fluorescence assay, and the results were analyzed with a mass-action model which could explain and predict the kinetics of fusion. The model accounted explicitly for the reduction in the fusion rate constant upon exposure of the virus to low pH, either for the virus alone in suspension or for the virus bound to the cells. When the pH was lowered without previous viral attachment to cells, an optimal fusion activity was detected at pH 5.2. When the virus was prebound to the cells, however, reduction of pH below 5.2 resulted in enhanced fusion activity at the initial stages. These results were explained by the fact that the rate constants of both fusion and inactivation increased severalfold at pH 4.5 or 4, compared to those at pH 5.2. At pH 5.2, lowering the temperature from 37 to 20 or 4 degrees C resulted in a decrease in the fusion rate constant by more than 30- or 1000-fold, respectively. Inactivation of the virus when preincubated in the absence of target membranes at pH 5 was found to be rapid and extensive at 37 degrees C, but was also detected at 0 degrees C. Our results indicate a strong correlation between fusion and inactivation rate ...Continue Reading

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Citations

Jun 21, 2005·Archives of Biochemistry and Biophysics·Austin BaileyLeonid V Chernomordik
Jan 4, 1998·Biochimica Et Biophysica Acta·J Ramalho-Santos, M C de Lima
Jun 6, 2000·Advanced Drug Delivery Reviews·N Düzgüneş, S Nir
Jul 17, 1998·Biochimica Et Biophysica Acta·J Ramalho-Santos, M C de Lima
Mar 1, 1995·Current Biology : CB·F M Hughson
Nov 2, 2002·The EMBO Journal·Eugenia LeikinaLeonid V Chernomordik
Aug 15, 2009·International Journal of Biological Sciences·Mohamed RajikKhatijah Yusoff
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C M CarrP S Kim
Nov 29, 2001·The Journal of Cell Biology·I MarkovicL V Chernomordik
Jan 3, 2015·Biochimica Et Biophysica Acta·Carmen L DelgadoFrancisco Gavilanes
Oct 10, 2006·Bioscience Reports·N Madhusudhana Rao, Vijaya Gopal
Feb 11, 2015·Archives of Virology·Ye ChenJingchen Sun
May 4, 2002·Biochimica Et Biophysica Acta·Isabel Nunes-CorreiaMaria C Pedroso de Lima
Aug 18, 2001·Biophysical Journal·S SchreiberH G Holzhütter
Jun 14, 1995·Biochimica Et Biophysica Acta·M C de LimaN Düzgüneş
Jul 13, 2016·Seminars in Cell & Developmental Biology·Jelle S BlijlevenAntoine M van Oijen
Mar 8, 2006·The Biochemical Journal·Ding-Kwo Chang, Shu-Fang Cheng
May 23, 2001·The Journal of General Virology·M J Edwards, N J Dimmock
Jan 8, 1999·Journal of Medical Primatology·E Mavoungou, V Poaty-Mavoungou
Jul 3, 2009·Environmental Science & Technology·David A GraiverShannon L Bartelt-Hunt
Oct 14, 1994·Science·C M Carr, P S Kim

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