A common structural motif in the binding of virulence factors to bacterial secretion chaperones

Molecular Cell
Mirjana LilicC Erec Stebbins

Abstract

Salmonella invasion protein A (SipA) is translocated into host cells by a type III secretion system (T3SS) and comprises two regions: one domain binds its cognate type III secretion chaperone, InvB, in the bacterium to facilitate translocation, while a second domain functions in the host cell, contributing to bacterial uptake by polymerizing actin. We present here the crystal structures of the SipA chaperone binding domain (CBD) alone and in complex with InvB. The SipA CBD is found to consist of a nonglobular polypeptide as well as a large globular domain, both of which are necessary for binding to InvB. We also identify a structural motif that may direct virulence factors to their cognate chaperones in a diverse range of pathogenic bacteria. Disruption of this structural motif leads to a destabilization of several chaperone-substrate complexes from different species, as well as an impairment of secretion in Salmonella.

References

Apr 1, 1996·Molecular Microbiology·P WattiauG R Cornelis
Jun 27, 2000·FEMS Microbiology Letters·M LermK Aktories
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G R Cornelis
Nov 14, 2000·Journal of Bacteriology·P A BronsteinS I Miller
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·M D WinnG N Murshudov
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·G Schiavo, F G van der Goot
Aug 17, 2001·Nature·C E Stebbins, J E Galán
May 15, 2002·International Journal of Medical Microbiology : IJMM·Michelle L ZaharikB Brett Finlay
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Mar 5, 2003·Current Opinion in Microbiology·Claude ParsotAnne-Laure Page
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·C Erec Stebbins, Jorge E Galán
Nov 19, 2003·Molecular Microbiology·Kumaran S Ramamurthi, Olaf Schneewind
Feb 6, 2004·Molecular Microbiology·Sang Ho Lee, Jorge E Galán
Feb 6, 2004·Journal of Bacteriology·Kristin EhrbarWolf-Dietrich Hardt
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Aug 31, 2004·Acta Crystallographica. Section D, Biological Crystallography·Jason PhanDavid S Waugh
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh
Feb 11, 2005·Journal of Molecular Biology·Florian D SchubotDavid S Waugh
Oct 7, 2005·Nature·Yukihiro Akeda, Jorge E Galán

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Citations

May 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michele LunelliMichael Kolbe
Apr 14, 2010·The Journal of Biological Chemistry·Viviana JobAndréa Dessen
Aug 16, 2011·The Journal of Biological Chemistry·Tara L ArchuletaBenjamin W Spiller
Nov 2, 2006·The Journal of Biological Chemistry·Steven JohnsonSusan M Lea
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Milos Vujanac, C Erec Stebbins
Oct 27, 2010·Infection and Immunity·Jason E McDermottFred Heffron
May 29, 2012·Journal of Bacteriology·Nikhil A ThomasCheryl Rafuse
Dec 31, 2008·Journal of Bacteriology·Hiroyuki Matsumoto, Glenn M Young
Dec 10, 2013·Nature Structural & Molecular Biology·Julia RadicsThomas C Marlovits
Apr 5, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Bharat G ReddyLawrence J DeLucas
Jul 9, 2014·Annual Review of Microbiology·Jorge E GalánSamuel Wagner
Feb 6, 2016·Journal of the American Chemical Society·Lun K TsouHoward C Hang
Nov 2, 2015·Trends in Biochemical Sciences·Athina G PortaliouAnastassios Economou
Dec 7, 2007·Journal of Molecular Biology·Carina R BüttnerHartmut H Niemann
Dec 28, 2007·Protein Expression and Purification·Ronald Raab, Wieslaw Swietnicki
Mar 26, 2016·Frontiers in Cellular and Infection Microbiology·F-X Campbell-Valois, Stéphanie M Pontier
Feb 14, 2009·Cellular Microbiology·Anastasia D GaziMichael Kokkinidis
Aug 9, 2008·Protein Science : a Publication of the Protein Society·Sigrun RumpelMarkus Zweckstetter
Aug 10, 2013·Cellular Microbiology·Nicola BarisonMichael Kolbe

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