A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer

The Review of Scientific Instruments
Osamu WakaoManabu Tokeshi

Abstract

Fluorescence polarization (FP) offers easy operation and rapid processing, making it implementable in molecular interaction analysis. Previously we have developed a unique FP measurement system using a liquid crystal (LC) layer and an image sensor. The system is based on a principle of synchronized detection between the switching rate of the LC layer and the sampling rate of the CCD. The FP system realized simultaneous multiple sample detection; however, the measurement precision was lower than that of the conventional FP apparatus. The main drawbacks were low light transmittance of the LC layer and insufficient synchronization between the LC layer and CCD. In this paper, we developed a new FP analyzer based on LC-CCD synchronization detection. By using a newly designed LC with high transmittance and improving synchronization, the performance of the system has been dramatically improved. Additionally, we reduced the cost by using an inexpensive CCD and an LED as the excitation source. Simultaneous FP immunoassay of multiple samples of prostaglandin E2 was performed. The error rate of the FP system is reduced from 16.9% to 3.9%, as comparable to the commercial conventional FP system.

References

Jun 1, 1996·Molecular Endocrinology·J R LundbladR H Goodman
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·D M Jameson, S E Seifried
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·J Lippincott-SchwartzA Kenworthy
Oct 25, 2001·Analytical Biochemistry·K KakehiM Kinoshita
Mar 2, 2002·Science·Hiroaki Kitano
Dec 20, 2002·Journal of Structural Biology·Merav CohenYosef Gruenbaum
Nov 5, 2003·Nature Biotechnology·Elizabeth A Jares-Erijman, Thomas M Jovin
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David M AronoffMarc Peters-Golden
Oct 25, 2008·Analytical Chemistry·Jorge A Cruz-Aguado, Gregory Penner
Aug 13, 2011·Analytical and Bioanalytical Chemistry·Tomoya TachiYoshinobu Baba

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