Jun 1, 1976

A compact form of double-stranded RNA in solutions containing poly(ethyleneglycol)

Nucleic Acids Research
Y M EvdokimovY M Varshavsky


Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regular internal structure, which accounts for the appearance of an intense positive band in CD spectra. Heating or acidification of PEG-containing solutions of dsRNA leads to the disappearance of the intense positive CD band, which results from the "destruction" of the regular internal structure of compact particles. Comparison of properties of DNA and dsRNA compact particles formed in PEG-containing water-salt solutions suggests the existence of similar mechanisms of compactization of double-stranded polynucleotides.

Mentioned in this Paper

RNA Conformation
RNA Denaturation
RNA, Double-Stranded
Acidification - ActCode
Ribosomal RNA
Sodium Chloride
Circular Dichroism, Vibrational

About this Paper

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.