A compact functional quantum Dot-DNA conjugate: preparation, hybridization, and specific label-free DNA detection

Langmuir : the ACS Journal of Surfaces and Colloids
Dejian ZhouDavid Klenerman

Abstract

In this letter, we report the preparation of a compact, functional quantum dot (QD)-DNA conjugate, where the capturing target DNA is directly and covalently coupled to the QD surface. This enables control of the separation distance between the QD donor and dye acceptor to within the range of the Förster radius. Moreover, a tri(ethylene glycol) linker is introduced to the QD surface coating to effectively eliminate the strong, nonspecific adsorption of DNA on the QD surface. As a result, this QD-DNA conjugate hybridizes specifically to its complementary DNA with a hybridization rate constant comparable to that of free DNAs in solution. We show this system is capable of specific detection of nanomolar unlabeled complimentary DNA at low DNA probe/QD copy numbers via a "signal-on" fluorescence resonance energy transfer (FRET) response.

References

Jul 18, 2001·Journal of the American Chemical Society·S PathakM E Thompson
Aug 28, 2003·Nature Materials·Igor L MedintzJ Matthew Mauro
Jan 6, 2004·Nature Biotechnology·Paul Alivisatos
Jan 8, 2004·Journal of the American Chemical Society·Aaron R ClappHedi Mattoussi
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·I L MedintzJ R Deschamps
Nov 5, 2004·Physical Review Letters·Zoher Gueroui, Albert Libchaber
Jan 18, 2005·Nucleic Acids Research·Michael M A SekarPamela M St John
Mar 18, 2005·Journal of the American Chemical Society·H Tetsuo UyedaHedi Mattoussi
May 11, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sungchul Hohng, Taekjip Ha
Aug 11, 2005·Journal of the American Chemical Society·Rumiana BakalovaYoshinobu Baba
Sep 30, 2005·Chemical Communications : Chem Comm·Dejian ZhouLiming Ying
Oct 29, 2005·Chembiochem : a European Journal of Chemical Biology·Matthew LevyAndrew D Ellington
Dec 22, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Aaron R ClappHedi Mattoussi
Dec 28, 2005·Nature Materials·Chun-Yang ZhangTza-Huei Wang
May 25, 2006·Journal of the American Chemical Society·Fang HeGuillermo C Bazan
Jul 18, 2006·Nature Reviews. Microbiology·David H J Bunka, Peter G Stockley
Aug 10, 2006·Journal of the American Chemical Society·Lifang ShiZeev Rosenzweig
Nov 23, 2006·Journal of the American Chemical Society·Thomas PonsHedi Mattoussi
Feb 24, 2007·Journal of the American Chemical Society·Hui PengJadranka Travas-Sejdic
Apr 5, 2007·Chemical Communications : Chem Comm·Maria Jose Ruedas-RamaElizabeth A H Hall
May 2, 2007·Journal of the American Chemical Society·Qiangbin WangHao Yan
May 29, 2007·Nano Letters·Igor L MedintzHedi Mattoussi
Jul 5, 2007·Journal of the American Chemical Society·Steven F Lee, Mark A Osborne
Oct 25, 2007·Journal of the American Chemical Society·Kimihiro SusumuHedi Mattoussi
Nov 15, 2007·Angewandte Chemie·Qiangbin WangHao Yan

❮ Previous
Next ❯

Citations

Jun 20, 2008·Analytical and Bioanalytical Chemistry·Kyle A CissellSapna K Deo
Mar 23, 2010·Analytical and Bioanalytical Chemistry·Feng ZhangWolfgang J Parak
Nov 17, 2011·Journal of the American Chemical Society·Moria KwiatFernando Patolsky
Feb 2, 2011·Chemical Communications : Chem Comm·Rohan T Ranasinghe, Tom Brown
Jul 26, 2013·Physical Chemistry Chemical Physics : PCCP·Somayeh Gholami, Mohsen Kompany-Zareh
Feb 22, 2012·Nanoscale Research Letters·Jin-Jin Li, Ka-Di Zhu
Jan 2, 2014·ACS Applied Materials & Interfaces·Shao SuLianhui Wang
Feb 13, 2010·Nanomedicine·Andrea StadlerOleg Gang
Sep 14, 2013·The Journal of Chemical Physics·Qun ZengJun Chen
Feb 19, 2010·Organic & Biomolecular Chemistry·Matthew BatchelorTrevor Rayment
Apr 7, 2009·Journal of Materials Chemistry·Zidong Wang, Yi Lu
Mar 24, 2012·Chemical Communications : Chem Comm·Haiyan Zhang, Dejian Zhou
Jul 21, 2015·Biosensors & Bioelectronics·Maja StanisavljevicVojtech Adam
Nov 23, 2015·Biosensors & Bioelectronics·Tzong-Yuan WuChung-Yung Chen
Mar 4, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Junghan LeeRita Song
Jul 29, 2010·Advanced Materials·Jing KangLars Dähne
Nov 28, 2012·Advanced Healthcare Materials·Lei SongDejian Zhou
Jul 24, 2012·Biochemical Society Transactions·Dejian Zhou
Dec 17, 2008·Physical Chemistry Chemical Physics : PCCP·Igor L Medintz, Hedi Mattoussi
Jul 22, 2010·Nanoscale·Yi-Ping Ho, Kam W Leong
Mar 18, 2011·Faraday Discussions·Haiyan ZhangDejian Zhou
Aug 30, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yiru PengJingyue Ju
Dec 4, 2013·Physical Chemistry Chemical Physics : PCCP·Christina Marie Tyrakowski, Preston Todd Snee
Jul 11, 2018·Materials·Luca GuerriniNicolas Pazos-Perez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.