A comparative analysis of FISH, RT-PCR, and cytogenetics for the diagnosis of bcr-abl-positive leukemias

American Journal of Clinical Pathology
M C CoxS Amadori

Abstract

Philadelphia (Ph) chromosome-positive leukemias, with the bcr-abl gene translocation, have a dismal prognosis. The identification of Ph-positive patients is vitally important because only aggressive therapeutic approaches, such as allogeneic bone marrow transplantation, may result in long-term disease-free survival. Routine diagnostic methods, such as Southern blot analysis and cytogenetics, may lead to false-negative results. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis is considered the most sensitive tool for the detection of the bcr-abl translocation, and it is widely used alone or in combination with karyotyping or Southern blot analysis to identify Ph-positive cases. In this study, we used fluorescence in situ hybridization (FISH) with BCR and ABL double-color probes for detecting Ph-positive leukemias. The FISH results were compared with the results of cytogenetic and RT-PCR analyses in 75 patients with leukemia or other myeloproliferative syndromes (chronic myeloid leukemia, 30; acute lymphoblastic leukemia, 24; acute myelogenous leukemia, 6; essential (hemorrhagic) thrombocythemia, 12; chronic myelomonocytic leukemia, 2; and polycythemia vera, 1). FISH analysis proved to be simple, extremely reliab...Continue Reading

Citations

Mar 5, 2003·Leukemia & Lymphoma·Catherine J WuJerome Ritz
Oct 31, 2009·Journal of Pediatric Hematology/oncology·Louise Olde NordkampHenk van den Berg
Feb 9, 2007·Molecular Diagnosis & Therapy·Sung-Min ChunByoung-Don Han
Sep 1, 2007·Expert Opinion on Medical Diagnostics·Brijesh Arora, Ayalew Tefferi
Feb 22, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Shuping YuHuimin Wang
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Shamima YeasminKohji Miyazaki
Jun 17, 2016·International Journal of Laboratory Hematology·R RanjbaranS Sharifzadeh
May 26, 1999·Leukemia & Lymphoma·H Avet-Loiseau
Apr 6, 2002·Clinics in Laboratory Medicine·Harold R SchumacherFermina Mazzella
Jan 13, 2018·Chemical Reviews·Alicia J AngelbelloMatthew D Disney

❮ Previous
Next ❯

Related Concepts

Related Feeds

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.