A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
J Souframanien, T Gopalakrishna

Abstract

Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in elite blackgram genotypes. A total of 25 random and 16 ISSR primers were used. Amplification of genomic DNA of the 18 genotypes, using RAPD analysis, yielded 104 fragments that could be scored, of which 44 were polymorphic, with an average of 1.8 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from two (OPA-13) to nine (OPK-4) and varied in size from 200 bp to 2,500 bp. Percentage polymorphism ranged from 16.6% (OPK-7) to a maximum of 66.6% (OPE-5, OPH-2, and OPK-8), with an average of 42.7%. The 16 ISSR primers used in the study produced 101 bands across 18 genotypes, of which 55 were polymorphic. The number of amplified bands varied from two (ISSR 858) to ten (ISSR 810), with a size range of 200-2,200 bp. The average numbers of bands per primer and polymorphic bands per primer were 6.3 and 3.4, respectively. Percentage polymorphism ranged from 25% (ISSR 885) to 100% (ISSR 858), with an average percentage polymorphism of 57.5% across all the genotypes. The 3'-anchored primers based on poly(GA) and poly(AG) motifs produced high average polymorphisms of 54.9...Continue Reading

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Citations

Aug 26, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B ChaitiengD A Vaughan
Jul 14, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y-Q LuC Preston
Jul 25, 2008·Genome Génome / Conseil National De Recherches Canada·S K GuptaT Gopalakrishna
Sep 7, 2007·Chinese Medicine·Pui Ying YipHoi Shan Kwan
Oct 20, 2012·Genetics and Molecular Research : GMR·P KhajudparnP A Tantasawat
Jun 9, 2011·Critical Reviews in Biotechnology·Maryam SarwatPrem Shankar Srivastava
Jun 13, 2015·Breeding Science·Anochar KaewwongwalPeerasak Srinives
Aug 12, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Zahra HajibaratReza Talebi
Aug 19, 2009·Molecular Biology Reports·D V N Sudheer PamidimarriMuppala P Reddy
Dec 1, 2015·Journal, Genetic Engineering & Biotechnology·A G A KhaledA A Said
Sep 8, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ouafae PakhrouBouchra Belkadi
Mar 12, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ruchi TyagiManjusha Verma
May 16, 2021·Scientific Reports·Ram Chandra Jena, Pradeep Kumar Chand

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