Jan 1, 1977

A comparative analysis of methods suitable for detection of platelet alloantibodies: inhibition of 14C-serotonin uptake, 14C-serotonin release and microcomplement fixation

Vox Sanguinis
D HeinrichC Mueller-Eckhardt

Abstract

Three HLA-specific antisera were comparatively analyzed by platelet microcomplement fixation and 14C-serotonin uptake and/or 14C-serotonin release studies with platelets. In general, there was a good correlation of HLA antibody specificity exhibited in all 3 test systems. Quantitative differences in the response of platelets to HLA antisera were observed and accounted for by differences in antigen density and different types of antibodies involved. Platelet autoantibodies could not be detected by means of 14C-serotonin release from platelets in plasma.

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Citations

Mentioned in this Paper

Autoantibodies
Immune Sera
Sample Fixation
Isoantibodies
HLA Antigens
5-hydroxytryptamine Uptake
Antigens
Metabolic Inhibition
Platelet Count Measurement
SD Antigens

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