A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone

Langenbeck's Archives of Surgery
Alexander E HandschinOmana A Trentz

Abstract

Heterotopic ossification (HO) is a pathological bone formation process in which ectopic bone is formed in soft tissue. The formation of bone depends on the expression of the osteoblast phenotype. Earlier studies have shown conflicting results on the expression of phenotype markers of cells originating from HO and normal bone. The hypothesis of the present study is that cells from HO show an altered expression of osteoblast-specific phenotype markers compared to normal osteoblasts. The aims of the study were to further characterize the expression of osteoblast phenotypemarkers and to provide a comparison with other study results. Using an in vitro technique, reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and immunohistochemistry, we compared the phenotype gene expression (type I collagen, alkaline phosphatase, Cbfa-1, osteocalcin) of osteoblasts from resected HO and normal bone (iliac crest). Cells from HO expressed the osteoblast phenotype (type I collagen, alkaline phosphatase) but were characterized by a depleted osteocalcin expression. The expression of Cbfa-1 (osteocalcin transcription gene) showed a large variety in our study. Preoperative radiotherapy had no effect on phenotype expression in cells...Continue Reading

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Citations

Apr 9, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·P ZermattenF Chevalley
Feb 15, 2008·Der Unfallchirurg·C DaubF W Thielemann
Oct 11, 2017·Journal of Pathology and Translational Medicine·Shipra Agarwal, Deepali Jain
May 6, 2017·Journal of Orthopaedic Surgery and Research·Laura E EdsbergJennifer T Wyffels

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