A comparative analysis of the bifunctional Cox proteins of two heteroimmune P2-like phages with different host integration sites

Virology
Alexandra Ahlgren-BergE Haggård-Ljungquist

Abstract

The Cox protein of the coliphage P2 is multifunctional; it acts as a transcriptional repressor of the Pc promoter, as a transcriptional activator of the P(LL) promoter of satellite phage P4, and as a directionality factor for site-specific recombination. The Cox proteins constitute a unique group of directionality factors since they couple the developmental switch with the integration or excision of the phage genome. In this work, the DNA binding characteristics of the Cox protein of WPhi, a P2-related phage, are compared with those of P2 Cox. P2 Cox has been shown to recognize a 9 bp sequence, repeated at least 6 times in different targets. In contrast to P2 Cox, WPhi Cox binds with a strong affinity to the early control region that contains an imperfect direct repeat of 12 nucleotides. The removal of one of the repeats has drastic effects on the capacity of WPhi to bind to the Pe-Pc region. Again in contrast to P2 Cox, WPhi Cox has a lower affinity to attP compared to the Pe-Pc region, and a repeat of 9 bp can be found that has 5 bp in common with the repeat in the Pe-Pc region. WPhi Cox, however, is essential for excisive recombination in vitro. WPhi Cox, like P2 Cox, binds cooperatively with integrase to attP. Both Cox prot...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S SahaK Nordström
Jun 1, 1987·Molecular & General Genetics : MGG·E Haggård-LjungquistL E Bertani
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Sep 1, 1965·Journal of General Microbiology·I Sasaki, G Bertani
Feb 1, 1997·Molecular Microbiology·M R ReedJ B Egan
Nov 14, 2000·Journal of Bacteriology·J M Eriksson, E Haggârd-Ljungquist
Jul 26, 2002·Molecular Microbiology·Ian B Dodd, J Barry Egan
Feb 13, 2003·Journal of Molecular Biology·John A Lewis, Graham F Hatfull
Sep 2, 2003·Molecular Biology and Evolution·Anders S NilssonElisabeth Haggård-Ljungquist
Feb 12, 2004·Genes & Development·Ian B DoddJ Barry Egan
Apr 7, 2004·Journal of Molecular Biology·My D SamRobert T Clubb
Jan 12, 2005·Nucleic Acids Research·Laura Pérez-LagoAna Camacho
Jan 22, 2005·Virology·Clara FrumerieElisabeth Haggård-Ljungquist
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sine L SvenningsenSankar Adhya
Aug 5, 2005·The Journal of Biological Chemistry·Latifa ElantakXavier Morelli
Nov 16, 2005·Annual Review of Genetics·Amos B OppenheimSankar Adhya
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mohamad A AbbaniRobert T Clubb
May 27, 2008·Journal of Bacteriology·Aras N MattisJeffrey F Gardner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.