A comparative analysis of the cross-reactivity in the polcalcin family including Syr v 3, a new member from lilac pollen

Allergy
A LedesmaR Rodríguez

Abstract

Polcalcins are pollen-specific allergens with two EF-hand calcium-binding sites that exhibit strong cross-reactivity. Our objective was to isolate and express the cDNA coding of the EF-hand calcium-binding allergen from lilac pollen and to study cross-reactivity with other polcalcins from related and nonrelated pollen sources with different specific antibodies and sera from two different populations. Specific cDNA was amplified by PCR, cloned and expressed in Escherichia coli. Purification was achieved by gel permeation and ion exchange chromatographies. ELISA titration and inhibition assays were performed using the recombinant forms of Syr v 3, Ole e 3, Che a 3 and Phl p 7 with sera from two Spanish regions with different sensitization profiles, as well as Phl p 7- and Ole e 3-specific polyclonal rabbit antisera, and an Ole e 3-specific monoclonal antibody. Syr v 3 displays two EF-hand consensus sites and 8863 Da of theoretical molecular mass. The allergen consists of 80 residues with identities ranging from 66 to 87% with polcalcins included in this study. Syr v 3, Ole e 3, Che a 3 and Phl p 7 showed a similar IgG- and IgE-binding capacity although differences at quantitative level were observed depending on the population of...Continue Reading

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Citations

Oct 4, 2007·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Richard W Weber
Jun 1, 2007·Nucleic Acids Research·Alvaro Martinez BarrioErik Bongcam-Rudloff
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Jun 19, 2014·The World Allergy Organization Journal·Barbara CasesEnrique Fernandez-Caldas
Aug 19, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D BarberG Salcedo
Feb 6, 2007·Immunology and Allergy Clinics of North America·Nicole WopfnerPeter Lackner
Dec 26, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rosalía RodríguezGuillermo Salamanca
Oct 31, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D BarberR Rodríguez
Jun 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R BarderasM Villalba
Aug 8, 2013·Methods : a Companion to Methods in Enzymology·Mayte VillalbaEva Batanero
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Jan 4, 2012·Annals of Nutrition & Metabolism·S Daniel Robert, Aziz Al-Safi Ismail
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra VerdinoWalter Keller
May 10, 2007·Current Opinion in Allergy and Clinical Immunology
Sep 3, 2018·International Archives of Allergy and Immunology·Carmen Oeo-SantosRodrigo Barderas

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