A comparative analysis of the proteins between the fibroblasts from Werner's syndrome patients and age-matched normal individuals using two-dimensional gel electrophoresis

Mechanisms of Ageing and Development
T TodaN Kimura

Abstract

Werner's syndrome (WS) is an autosomal recessive disorder causing symptoms of premature aging. The fibroblasts of WS patients have a shorter life-span than normal fibroblasts. We analyzed the fibroblast proteins from three WS patients and from three age-matched normal individuals using two-dimensional gel electrophoresis and image processing. The expressions of 12 proteins were shown to be augmented or suppressed in WS fibroblasts compared with normal fibroblasts: 11 of 12 spots on the electrophoresis gel of WS fibroblasts were denser than the corresponding spots of normal individual fibroblasts, while the remaining one spot was fainter in WS fibroblasts than in normal fibroblasts. The abundance of these proteins were compared to those of the corresponding proteins from normal fibroblasts at various cell passages in vitro reported in the TMIG-2DPAGE database. The result shows that the change in the protein patterns in in vitro aging did not necessarily correspond to the change in WS fibroblasts, except for three proteins abundant in WS fibroblasts, which increased their abundance during in vitro aging. These results suggest that the premature aging process of WS fibroblasts shares only part of the in vitro aging process of norm...Continue Reading

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Citations

Jul 25, 2000·Mechanisms of Ageing and Development·I OhsugiY Furuichi
Jan 17, 2002·Journal of Biotechnology·Rashmi KorkeWei Shou Hu
Mar 23, 2002·Experimental Gerontology·Jean-François DierickOlivier Toussaint
Aug 30, 2008·Rejuvenation Research·Satoshi HonjoYasushi Saito
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kasper J KyngVilhelm A Bohr
Jun 30, 2016·Ageing Research Reviews·João Pinto da CostaTeresa Rocha-Santos
Jul 27, 2000·Annals of the New York Academy of Sciences·J O NehlinV A Bohr
Jan 25, 2002·Annals of the New York Academy of Sciences·T Toda
Feb 13, 1999·Cell·F B JohnsonL Guarente

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