A comparative analysis of the response of the hepatic transcriptome to dietary docosahexaenoic acid in Atlantic salmon (Salmo salar) post-smolts

BMC Genomics
Brett D GlencrossDouglas R Tocher

Abstract

The present study aimed to explore the impact of dietary docosahexaenoic acid (DHA) on aspects of the metabolism of Atlantic salmon (Salmo salar). The effects of diets containing increasing levels of DHA (1 g kg(-1), 3 g kg(-1), 6 g kg(-1), 10 g kg(-1) and 13 g kg(-1)) on the liver transcriptome of post-smolt salmon was examined to elucidate patterns of gene expression and responses of specific metabolic pathways. Total RNA was isolated from the liver of individual fish and analyzed using a custom gene expression 44K feature Atlantic salmon oligo-microarray. The expression of up to 911 unique annotated genes was significantly affected by dietary DHA inclusion relative to a low DHA reference diet. Analysis of a total of 797 unique genes were found with a significant linear correlation between expression level and dietary DHA. Gene-Set Enrichment Analysis (GSEA) identified a range of pathways that were significantly affected by dietary DHA content. Pathways that showed a significant response to dietary DHA level included those for long-chain polyunsaturated fatty acid biosynthesis, fatty acid elongation, steroid biosynthesis, glycan biosynthesis, protein export and protein processing in the endoplasmic reticulum. These findings s...Continue Reading

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Citations

May 29, 2018·Animal Science Journal = Nihon Chikusan Gakkaihō·Ahmed A ElolimyJuan J Loor
Nov 23, 2018·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Houguo XuMengqing Liang

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Datasets Mentioned

BETA
E-MTAB-3180

Methods Mentioned

BETA
electrophoresis
Feature Extraction
protein-folding

Software Mentioned

Limma
R
GAGE ( Generally Applicable Gene - set Enrichment [
Agilent Feature Extraction
GSEA
KAAS
Bioconductor
lmFit
BLAST

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