A comparative assessment of cigarette smoke aerosols using an in vitro air-liquid interface cytotoxicity test

Inhalation Toxicology
David ThorneClive Meredith

Abstract

This study describes the evaluation of a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. The functionality and applicability of this modified protocol was assessed by comparing the cytotoxicity profiles from eight different cigarettes. Three reference cigarettes, 1R5F, 3R4F and CORESTA Monitor 7 were used to put the data into perspective and five bespoke experimental products were manufactured, ensuring a balanced and controlled study. Manufactured cigarettes were matched for key variables such as nicotine delivery, puff number, pressure drop, ventilation, carbon monoxide, nicotine free dry particulate matter and blend, but significantly modified for vapor phase delivery, via the addition of two different types and quantities of adsorptive carbon. Specifically manufacturing products ensures comparisons can be made in a consistent manner and allows the research to ask targeted questions, without confounding product variables. The results demonstrate vapor-phase associated cytotoxic effects and clear differences between the products tested and their cytotoxic profiles. This study has further characterized the in vitro vapor phase biological response relationship and conf...Continue Reading

References

Mar 1, 1997·Journal of Toxicology and Environmental Health·D Hoffmann, I Hoffmann
Nov 5, 1997·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·D W BombickD J Doolittle
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Cristina AndreoliAlfredo Nunziata
Apr 20, 2004·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yasuo FukanoMutsuaki Suzuki
Sep 27, 2005·Alternatives to Laboratory Animals : ATLA·Jeremy PhillipsEian Massey
Aug 30, 2007·The Lancet Oncology·Yann GrosseUNKNOWN WHO International Agency for Research on Cancer Monograph Working Group
Feb 22, 2008·Regulatory Toxicology and Pharmacology : RTP·Christopher R E Coggins, Charles L Gaworski
Nov 26, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Han-Jae ShinHak-Chul Hyun
Mar 19, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chuan LiuChristopher Proctor
Apr 20, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K G McAdamC J Proctor
Mar 8, 2013·Tobacco Control·Maciej Lukasz GoniewiczNeal Benowitz
Jul 16, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·David Thorne, Jason Adamson
Jul 6, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J AdamsonC Meredith
Aug 12, 2014·SpringerPlus·David J DittrichChristopher J Proctor
Jun 23, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·David AzzopardiGary Phillips

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Citations

Jul 30, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xiang Li
Sep 11, 2016·Particle and Fibre Toxicology·John W WillsShareen H Doak
Nov 1, 2016·Toxicology Mechanisms and Methods·David AzzopardiMarianna Gaça
Oct 17, 2019·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dana LautersteinR Philip Yeager
Jun 5, 2018·Chemical Research in Toxicology·Shigehisa UchiyamaNaoki Kunugita

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Minitab®
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