A comparative biochemical and immunological analysis of cytokeratin patterns in the oral epithelium of the miniature pig and man.

Archives of Oral Biology
A H ShabanaN Forest

Abstract

In man, cytokeratin constitutes a family of 19 polypeptides that show different but distinct distribution patterns in the various epithelia. Changes in these patterns may occur during epithelial development and differentiation. The cytokeratin patterns in the oral mucosa of the miniature pig, an animal used in studies of wound healing, were investigated. Surgical biopsies were obtained from the gingiva, hard palate and alveolar mucosa of both man and pig. The cytokeratins were analysed by immunofluorescence, two-dimensional gel electrophoresis and by immunoblotting. Nine monoclonal antibodies were used to identify the different cytokeratin polypeptides in cryostat sections. Two-dimensional gel electrophoresis showed that pig oral mucosa contains at least 10 different polypeptides, five of the acidic type I and five of the basic type II cytokeratins. These were different from the human cytokeratin polypeptides and accordingly were designated P1-P10, according to their molecular weight and isoelectric mobility. Their molecular weight varied between 48 and 69 kdalton and the pHi varied between 5 and 7.3. Immunoblotting showed the monoclonal antibody Ks 13.1 (anticytokeratins Nos 13 and 14) to cross-react with the pig polypeptides ...Continue Reading

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Citations

Oct 1, 1990·The American Journal of Anatomy·M H SawafN Forest
May 1, 1994·European Journal of Cancer. Part B, Oral Oncology·P R Morgan, L Su
Jan 30, 2004·Oral Oncology·Ojaswini S UpasaniAvinash N Bhisey
Sep 6, 2000·Archives of Oral Biology·A W BarrettB K Berkovitz
Nov 25, 1998·Journal of Anatomy·A W BarrettB K Berkovitz
Aug 1, 1994·Scandinavian Journal of Dental Research·H MaedaP Holmstrup
Feb 20, 1998·European Journal of Oral Sciences·M Feghali-AssalyJ P Ouhayoun
Sep 1, 1990·Journal of Periodontal Research·J P OuhayounN Forest
Nov 1, 1992·Journal of Periodontal Research·A PelissierN Forest
May 1, 1994·Journal of Periodontal Research·M Feghali-AssalyJ P Ouhayoun
May 17, 2002·The Bulletin of Tokyo Dental College·Kazuhiro FukumashiTakashi Inoue
Apr 24, 2004·The Bulletin of Tokyo Dental College·Masatsugu FujisekiTakashi Inoue
Oct 4, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Guoliang SaYifang Zhao
Jan 1, 1991·Archives of Oral Biology·A H ShabanaN Forest
Feb 10, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Celia Carrillo GarcíaFrancisco Vera Sempere
Jan 1, 1989·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·F X BoschW W Franke

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