PMID: 2119175Sep 1, 1990Paper

A comparative biochemical and ultrastructural study of proteoglycan-collagen interactions in corneal stroma. Functional and metabolic implications

The Biochemical Journal
J E Scott, T R Bosworth

Abstract

1. Corneas of mouse, rat, guinea pig, rabbit, sheep, cat, dog, pig and cow were quantitatively analysed for water, hydroxyproline, nucleic acid, total sulphated polyanion, chondroitin sulphate/dermatan sulphate and keratan sulphate, several samples or pools of tissue from each species being used. Ferret cornea was similarly analysed for water and hydroxyproline on one pool of eight corneas. Pooled frog (38) and ferret (eight) corneas and a single sample of human cornea were qualitatively examined for keratan sulphate and chondroitin sulphate/dermatan sulphate by electrophoresis on cellulose acetate membranes. Nine species (mouse, frog, rat, guinea pig, rabbit, sheep, cat, pig and cow) were examined by light microscopy and six (mouse, frog, rat, guinea pig, rabbit and cow) by electron microscopy, with the use of Alcian Blue or Cupromeronic Blue in critical-electrolyte-concentration (CEC) methods to stain proteoglycans. 2. Water (% of wet weight), hydroxyproline (mg/g dry wt.) and chondroitin sulphate (mg/g of hydroxyproline) contents were approximately constant across the species, except for mouse. 3. Keratan sulphate contents (mg/g of hydroxyproline) increased with corneal thickness, whereas dermatan sulphate contents decreased...Continue Reading

Citations

Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C X CaiT F Linsenmayer
May 19, 2005·Biology of the Cell·Gianluca TettamantiMagda de Eguileor
Jul 7, 2015·Progress in Retinal and Eye Research·Keith M Meek, Carlo Knupp
Dec 18, 2013·Experimental Eye Research·Hamed Hatami-Marbini, Abdolrasol Rahimi
Jan 27, 1998·Experimental Eye Research·H GongT F Freddo
Jul 1, 2016·Journal of the Royal Society, Interface·Barbara J MurienneThao D Nguyen
Jul 20, 2018·Investigative Ophthalmology & Visual Science·Dan E MidgettThao D Nguyen
Oct 25, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Sally HayesKeith M Meek
Mar 10, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew J QuantockTomoya O Akama
Jun 20, 2013·Analytical and Bioanalytical Chemistry·Giulia VeronesiAndrew J Quantock
Mar 29, 2014·Annals of Biomedical Engineering·Hamed Hatami-Marbini
Jul 28, 2013·Current Eye Research·Hamed Hatami-MarbiniAbdolrasol Rahimi
Mar 3, 2017·BioMed Research International·Mariam Lotfy KhaledYutao Liu
Jul 4, 2012·Biologie aujourd'hui·Jacqueline Labat-RobertLadislas Robert
Jul 16, 2005·Current Eye Research·Rejane M GóesEduardo M Laicine
Jul 6, 2010·Current Eye Research·Barbara P PalkaAndrew J Quantock
Jun 9, 2017·Journal of the Royal Society, Interface·Sally HayesKeith M Meek
Mar 10, 1994·Biochimica Et Biophysica Acta·G E Ysart, R M Mason
Sep 1, 1992·Trends in Biochemical Sciences·J E Scott
Jun 17, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·A M HockingD J McQuillan
Apr 27, 2004·Experimental Eye Research·Jorge Fischbarg, David M Maurice

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