PMID: 2415918Oct 1, 1985Paper

A comparative electrophysiological study of enzymatically isolated single cells and strips of frog ventricle

Pflügers Archiv : European journal of physiology
L Tung, M Morad

Abstract

Single heart cells were obtained from frog ventricle using an enzymatic dispersion technique. The whole cell variation of the patch clamp technique was used to monitor action potential and cell membrane currents. The clamp circuit could be switched electronically between voltage and current clamp modes. The effects of seal leakage currents were to depolarize the cell, reduce the amplitude of the plateau, and lengthen the action potential duration. A scheme to compensate for these currents is presented. The membrane currents obtained from the single cell under voltage clamp conditions were compared to those obtained from multicellular preparations using the single sucrose gap technique. Hyperpolarizing clamps showed time-dependent, depletion-related K+ currents for the multicellular preparation, whereas for the single cell no such currents were observed. The absence of extracellular accumulation or depletion of K+ in the single cell was confirmed by the lack of post-clamp afterpotentials or changes in resting potential following a train of frequently elicited action potentials. The TTX-insensitive inward current was relatively faster in the single cell, compared to that measured in the multicellular preparation. A delayed time-d...Continue Reading

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Citations

Oct 1, 1989·Pflügers Archiv : European journal of physiology·V J Schouten, M Morad
Apr 1, 1992·Pflügers Archiv : European journal of physiology·M NeunlistL Tung
Dec 1, 1986·Pflügers Archiv : European journal of physiology·G Champigny, J Lenfant
Oct 1, 1994·Pacing and Clinical Electrophysiology : PACE·M Neunlist, L Tung
Jan 1, 1987·Fundamental & Clinical Pharmacology·P CharnetJ Nargeot
Nov 14, 2015·Circulation Research·Josefina Ramos-FrancoAriel L Escobar
Sep 1, 1987·The American Journal of Physiology·M LiebermanM Poenie
Jan 22, 1998·The American Journal of Physiology·M Neunlist, L Tung

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