A comparative evaluation of molecular recognition by monolayers composed of synthetic receptors or oriented antibodies

Biosensors & Bioelectronics
Inger Vikholm-LundinKirsi Tappura

Abstract

Recombinant anti-morphine Fab' fragments have been immobilised on gold by covalent attachment through the free thiol groups of the fragment. The antibody fragments were intercalated with a non-ionic hydrophilic polymer in order to suppress non-specific binding of interfering substances. The antibodies are oriented on the surface due to the thiol groups of the antibody and the layer shows a high response to antigen. Non-specific binding of bovine serum albumin is moreover very low because of the repellent polymer. Synthetic receptors composed of an imprinted self-assembled monolayer made from lipoates and the template, morphine, exhibit the same binding response to the antigen, morphine as the site-specific oriented antibody monolayer. A similar binding curve could be obtained as that for binding of morphine to an antibody Fab' fragment/polymer layer - indicating that synthetic receptors produced are comparable to those of antibody layers. Concentrations down to 0.1ng/ml have been measured with surface plasmon resonance.

References

Feb 18, 1993·Nature·G VlatakisK Mosbach
May 21, 2005·Biosensors & Bioelectronics·Inger Vikholm-Lundin, Willem M Albers
Jun 29, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Inger Vikholm-Lundin

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Citations

Nov 1, 2011·Journal of Molecular Recognition : JMR·Subramanian Balamurugan, David A Spivak
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Mar 31, 2015·Biosensors & Bioelectronics·Victor Crivianu-Gaita, Michael Thompson
Mar 2, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Doudou LouYu Zhang

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