A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes

Veterinary Parasitology
W G HollandJ Vercruysse

Abstract

In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. The combined estimates of sensitivity (CE(se)) of the PCR proved to be highest at 78.2%, closely followed by the mouse inoculation (MI), the micro-haematocrite centrifugation technique (MHCT) and the mini-anion-exchange centrifugation technique (MAECT) with CE(se) of, respectively, 74.0, 69.6 and 62.4%. The CE(se) of the buffy-coat technique (BCT) at 38.6% and the sodium dodecyl sulfate (SDS) clarification technique at 25.1% were considerably lower. PCR detected consistently all buffaloes infected from week 3 post-infection (PI) onwards. For MI this occurred after 5 weeks PI while for MHCT and MAECT these sustainable high levels were reached in the 7th week PI. BCT and SDS never detected all buffaloes infected. The influence of time and temperature on the viability of T. evansi in heparinized blood from water buffalo was also studied. In general we observed that the survival time tends to be longer when blood is kept at 4 degrees C. In samples kept in direct sunlight parasites became undetectable with the MHCT af...Continue Reading

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Citations

Jun 26, 2008·The Journal of Parasitology·Mrityunjoy MandalNihar Kanta Sasmal
Jun 15, 2010·Research in Veterinary Science·Kaio César Simano TavaresLuiz Claudio Miletti
Jun 1, 2010·The Lancet Infectious Diseases·Stijn Deborggraeve, Philippe Büscher
Sep 24, 2015·Acta Tropica·Norbert Georg SchwarzHagen Frickmann
Dec 18, 2004·Annals of the New York Academy of Sciences·Carlos GutierrezPhilippe Büscher
Oct 11, 2005·Journal of Comparative Pathology·A P DargantesD B Copeman
Jun 14, 2005·Annals of Tropical Medicine and Parasitology·H GarciaA Mendoza-Leon
Jan 12, 2013·Veterinary Parasitology·G R RudramurthyH Rahman
Sep 4, 2019·The Onderstepoort Journal of Veterinary Research·Tahani S BehourSaeed A El-Ashram
Nov 17, 2012·Expert Review of Molecular Diagnostics·Stijn Deborggraeve, Philippe Büscher

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