A comparative evaluation of the sensitivity of two automated and two manual nucleic acid extraction methods for the detection of norovirus by RT-PCR

Journal of Virological Methods
Kristie J WitloxJohn Marshall

Abstract

The aim of the study was to compare the sensitivity of a norovirus RT-PCR method using two manual RNA extraction methods (Qiagen and Roche) and two automated RNA extraction methods (Qiagen and Corbett). All four RNA extraction methods gave similar sensitivities although the automated methods, especially the Corbett, required significantly less labour than the manual methods. The automated methods also enabled RNA extraction of approximately two to three times the number of specimens in a given time period compared to manual methods.

References

Sep 9, 2004·Current Opinion in Infectious Diseases·Benjamin Clark, Mike McKendrick
Jan 5, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J A MarshallG G Hogg

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Citations

Feb 16, 2013·Food and Environmental Virology·Sara RomaniMohammad Reza Zali
Aug 19, 2010·International Journal of Environmental Research and Public Health·Leesa D Bruggink, John A Marshall
Apr 29, 2014·Diagnostic Microbiology and Infectious Disease·N L DunbarJ A Marshall
Nov 3, 2009·Journal of Virological Methods·Naoyuki NishimuraSetsuko Ishida
Oct 24, 2008·Diagnostic Microbiology and Infectious Disease·Kristie J WitloxJohn A Marshall
Apr 17, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jens VerheyenHarald H Kessler
Oct 28, 2008·Journal of Virological Methods·Thomas Bruun RasmussenDonald P King
Jul 25, 2009·Journal of Medical Virology·L D Bruggink, J A Marshall
Mar 19, 2015·Journal of Medical Virology·Leesa D BrugginkJohn A Marshall
Feb 4, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·L D BrugginkJ A Marshall
Mar 8, 2016·Journal of Medical Virology·Leesa D BrugginkJohn A Marshall
Jul 26, 2017·Journal of Medical Microbiology·Leesa D BrugginkJohn A Marshall
Dec 19, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Chantal ReuskenRemi N Charrel

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