A comparative fluorescent beacon-based method for serum microRNA quantification

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Madhu BetaJanakiraman Narayanan

Abstract

Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.

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Citations

Mar 13, 2017·Colloids and Surfaces. B, Biointerfaces·Minyoung JooTae Jung Park
Oct 2, 2017·Biomedical Microdevices·Bindu SalimThiagarajan Sairam
Sep 12, 2018·International Journal of Molecular Sciences·Jaqueline Carvalho de OliveiraMaría Sol Brassesco

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