A comparative GC-MS analysis of bioactive secondary metabolites produced by halotolerant Bacillus spp. isolated from the Great Sebkha of Oran.

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Fatima NasNihel Klouche-Khelil

Abstract

The reemergence of infectious diseases and resistant pathogens represents a serious problem for human life. Hence, the screening for new or alternative antimicrobial compounds is still urgent. Unusual ecosystems such as saline habitats are considered promising environments for the purposes of isolating bacterial strains able to produce potent natural products. The aim of this study is the identification of bioactive compounds biosynthesized by three halotolerant strains isolated from the Sebkha of Oran (Algeria) using gas chromatography coupled to mass spectrometry. Primary screening investigation of antimicrobial activities were performed against reference bacterial and fungal strains and revealed a broad-spectrum activity. Secondary metabolite extraction was carried out using ethyl acetate and chloroform. Crude extracts were tested for bioactivity using the disc diffusion method and subjected to GC-MS analysis. The extracts showed an important inhibitory effect against all tested strains. Fifty-six compounds were identified; they include tert-butyl phenol compounds, fatty acid methyl esters due to the methylation procedure, hydrocarbons, aldehydes, benzoquinones, pyrrols, and terpenes. Literature reports such compounds to hav...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1984·Journal of General Microbiology·N A Logan, R C Berkeley
Jun 28, 2001·Journal of Clinical Microbiology·J L Rodríguez-TudelaE Mellado
Jul 3, 2007·Microbial Ecology·Amarja A JoshiSeema S Sarnaik
Dec 25, 2007·Extremophiles : Life Under Extreme Conditions·Koki Horikoshi
Jul 2, 2009·International Journal of Systematic and Evolutionary Microbiology·N A LoganA Ventosa
Jul 23, 2009·Expert Review of Anti-infective Therapy·Emilia CantónJavier Pemán
Nov 4, 2009·Current Protocols in Microbiology·Jackie ReynoldsDonald P Breakwell
May 27, 2010·Indian Journal of Pharmaceutical Sciences·U S MishraB S Naik
Feb 22, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Heather Maughan, Geraldine Van der Auwera
May 17, 2011·International Journal of Systematic and Evolutionary Microbiology·M BagheriA Ventosa
Jul 19, 2011·Applied and Environmental Microbiology·Atika MeklatAhmed Lebrihi
Aug 15, 2013·Marine Drugs·Muhammad Abdul Mojid MondolMohammad Tofazzal Islam
Nov 22, 2013·Inhalation Toxicology·Valerie E MeyersJohn T James
Jul 15, 2015·International Journal of Food Microbiology·Kontham Kulangara VarshaKesavan Madhavan Nampoothiri
Aug 15, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Tarik AannizMohamed Amar
Dec 23, 2016·International Journal of Systematic and Evolutionary Microbiology·Seok-Hwan YoonJongsik Chun
Apr 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ramganesh SelvarajanStephen Meddows-Taylor
Apr 1, 2016·Journal of Pharmaceutical Analysis·Mounyr BalouiriSaad Koraichi Ibnsouda
Mar 13, 2018·FEMS Microbiology Reviews·Nina Gunde-CimermanAharon Oren
Mar 31, 2018·Genome Announcements·Mohamed Seghir DaasJohn C Vederas
Mar 16, 2019·Frontiers in Microbiology·Simon CaulierJacques Mahillon
May 28, 2019·World Journal of Microbiology & Biotechnology·Caroline F Ajilogba, Olubukola O Babalola
Aug 23, 2019·World Journal of Microbiology & Biotechnology·Taha MenasriaMargarita Aguilera

❮ Previous
Next ❯

Datasets Mentioned

BETA
LMB3093
MN519532

Methods Mentioned

BETA
electrophoresis
scraping
PCR

Software Mentioned

EzBioCloud
ANOVA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.