A comparative gene analysis with rice identified orthologous group II HKT genes and their association with Na(+) concentration in bread wheat

BMC Plant Biology
H A Chandima K AriyarathnaMichael G Francki

Abstract

Although the HKT transporter genes ascertain some of the key determinants of crop salt tolerance mechanisms, the diversity and functional role of group II HKT genes are not clearly understood in bread wheat. The advanced knowledge on rice HKT and whole genome sequence was, therefore, used in comparative gene analysis to identify orthologous wheat group II HKT genes and their role in trait variation under different saline environments. The four group II HKTs in rice identified two orthologous gene families from bread wheat, including the known TaHKT2;1 gene family and a new distinctly different gene family designated as TaHKT2;2. A single copy of TaHKT2;2 was found on each homeologous chromosome arm 7AL, 7BL and 7DL and each gene was expressed in leaf blade, sheath and root tissues under non-stressed and at 200 mM salt stressed conditions. The proteins encoded by genes of the TaHKT2;2 family revealed more than 93 % amino acid sequence identity but ≤52 % amino acid identity compared to the proteins encoded by TaHKT2;1 family. Specifically, variations in known critical domains predicted functional differences between the two protein families. Similar to orthologous rice genes on chromosome 6L, TaHKT2;1 and TaHKT2;2 genes were loca...Continue Reading

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Citations

May 21, 2016·Genome Génome / Conseil National De Recherches Canada·H A Chandima K Ariyarathna, Michael G Francki
Dec 6, 2017·The New Phytologist·Yongqing Yang, Yan Guo
Aug 1, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Muhammad A AsifAllison S Pearson
Mar 25, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jafar AhmadiPeter Poczai

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Datasets Mentioned

BETA
KR422355
KR422356
KR422357
KR422358
AJ491819
KJ540618

Methods Mentioned

BETA
PCR
protein folding
electrophoresis

Software Mentioned

Align BLAST
molecular Graphics system
PyMOL viewer
NetPrimer
GENEIOUS
PLACE
RepeatMasker
blastn
Interpro Protein Domains search
TREP

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