A comparative in vitro study of the toxic potency of five inorganic lead compounds on a rat liver epithelial cell line (REL)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Maria Francesca AleoGiovanna Mazzoleni

Abstract

Relative insolubility of inorganic Pb compounds is one of the major problems in the evaluation of the toxicological profile of this metal. Different characteristics of Pb-containing solutions may, in fact, alter the biological properties of Pb compounds and influence their toxic potency. To investigate these aspects, we used selected experimental conditions to evaluate and compare the specific biological effects of five inorganic Pb compounds (soluble salts and oxide) on the viability and proliferation rate of a rat liver-derived cell line (REL cells). The study was performed according to classical toxicological criteria (dose- and time-response, reversibility/transience of the effect). Each Pb compound was accurately solubilised and the quantification of the real concentration of Pb(II) ions was performed either on the culture media used for each treatment, or on the extracts of exposed cells. Our study shows that four, out of the five Pb compounds we tested, induce the same dose- and time-related anti-proliferative effects on REL cells, being these effects also reversible, transient and directly related to the intracellular content of the metal. Since the intracellular concentration of the metal and, consequently, its biologi...Continue Reading

References

Mar 1, 1989·Toxicology Letters·R A Goyer
Oct 1, 1988·Carcinogenesis·J T ZelikoffT G Rossman
Aug 15, 2000·American Journal of Industrial Medicine·P ApostoliG Mazzoleni
Oct 29, 2005·FEBS Letters·Roberta GiulianiMaria Francesca Aleo

❮ Previous
Next ❯

Citations

Oct 24, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F RovettaG Mazzoleni
Dec 30, 2011·Aquatic Toxicology·Sandrine PereiraChristelle Adam-Guillermin
Apr 7, 2007·Alternatives to Laboratory Animals : ATLA·Małgorzata Witeska, Marta Wakulska
Oct 13, 2018·Toxicology Letters·Piyawan PaisrisarnSuwimon Boonrungsiman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis