A comparative molecular and 3-dimensional structural investigation into cross-continental and novel avian Trypanosoma spp. in Australia

Parasites & Vectors
Crystal CooperPeta L Clode

Abstract

Molecular and structural information on avian Trypanosoma spp. throughout Australia is limited despite their intrinsic value in understanding trypanosomatid evolution, diversity, and structural biology. In Western Australia tissue samples (n = 429) extracted from 93 birds in 25 bird species were screened using generic PCR primers to investigate the diversity of Trypanosoma spp. To investigate avian trypanosome structural biology the first 3-dimensional ultrastructural models of a Trypanosoma spp. (Trypanosoma sp. AAT) isolated from a bird (currawong, Strepera spp.) were generated using focussed ion beam milling combined with scanning electron microscopy (FIB-SEM). Here, we confirm four intercontinental species of avian trypanosomes in native Australian birds, and identify a new avian Trypanosoma. Trypanosome infection was identified in 18 birds from 13 different bird species (19%). A single new genotype was isolated and found to be closely related to T. culicavium (Trypanosoma sp. CC2016 B002). Other Trypanosoma spp. identified include T. avium, T. culicavium, T. thomasbancrofti, Trypanosoma sp. TL.AQ.22, Trypanosoma sp. AAT, and an uncharacterised Trypanosoma sp. (group C-III sensu Zidková et al. (Infect Genet Evol 12:102-112,...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y TzfatiJ Shlomai
Nov 25, 1986·Nucleic Acids Research·A González
Feb 1, 1973·The Journal of protozoology·L Simpson
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M BarroisF Galibert
Jul 15, 1999·Experimental Parasitology·V YurchenkoJ Lukes
Jun 3, 2000·Parasitology Research·K MirandaW de Souza
Feb 13, 2001·Experimental Parasitology·J Lukes, J Votýpka
Nov 29, 2002·Eukaryotic Cell·Julius LukesPaul T Englund
Jul 17, 2003·International Journal for Parasitology·Elielton R CoelhoRosane Silva
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jan 1, 1991·Parasitology Today·V Apanius
Jan 15, 2005·Protist·Kildare MirandaWanderley de Souza
Mar 2, 2005·Nature Reviews. Microbiology·Roberto DocampoSilvia N J Moreno
Mar 22, 2005·International Journal for Parasitology·P B HamiltonW C Gibson
May 10, 2006·Molecular and Biochemical Parasitology·Frédéric BringaudVirginie Coustou
Oct 7, 2006·Biochimica Et Biophysica Acta·Paul A M MichelsVéronique Hannaert
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Neta MilmanJoseph Shlomai
Apr 10, 2008·Molecular Biology and Evolution·David Posada
May 3, 2008·Microscopy Research and Technique·Celso Sant'AnnaNarcisa L Cunha e Silva
Jul 29, 2008·Memórias do Instituto Oswaldo Cruz·Wanderley de Souza
Apr 22, 2009·The International Journal of Biochemistry & Cell Biology·Wanderley de SouzaJuliany C F Rodrigues
Mar 12, 2010·RNA Biology·Stephen Hajduk, Torsten Ochsenreiter
Apr 26, 2011·International Journal of Systematic and Evolutionary Microbiology·Jan VotýpkaMilena Svobodová
Apr 27, 2011·Parasitology·J M AustenS A Reid
May 14, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Thiago Cesar Prata RamosSergio Schenkman
Nov 15, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Lenka ZídkováMilena Svobodová
Aug 9, 2012·Histochemistry and Cell Biology·Wendell Girard-DiasKildare Miranda
Aug 13, 2013·Acta Tropica·Miriam Pires de Castro OliveiraEdna Freymuller Haapalainen
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 13, 2014·Journal of Cell Science·Carolina L AlcantaraNarcisa L Cunha-e-Silva
Jun 4, 2014·Molecular and Biochemical Parasitology·Julius LukešVyacheslav Yurchenko
Nov 30, 2014·Journal of Structural Biology·Caroline KizilyaprakBruno M Humbel

❮ Previous
Next ❯

Citations

Sep 16, 2020·The Journal of Parasitology·Monique L WinterhoffKevin C Rowe
Jun 24, 2019·Parasitology Research·Pornchai PornpanomMalisa Santavakul
Mar 9, 2019·International Journal for Parasitology. Parasites and Wildlife·Marina Silva RodriguesAna Maria Jansen

❮ Previous
Next ❯

Datasets Mentioned

BETA
M17995
M18814
KY425593
KY425594
KY498637

Methods Mentioned

BETA
transmission electron microscopy
imaging techniques
scanning electron microscopy
PCR
electron microscopy

Software Mentioned

MEGA
jModelTest
Geneious
MUSCLE
Amira
Mr Bayes

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.