A Comparative Study Between the Antibacterial Effect of Nisin and Nisin-Loaded Chitosan/Alginate Nanoparticles on the Growth of Staphylococcus aureus in Raw and Pasteurized Milk Samples

Probiotics and Antimicrobial Proteins
Maryam ZohriSeyed Kazem Sadjadi

Abstract

The aim of this study was to evaluate the antibacterial effect of nisin-loaded chitosan/alginate nanoparticles as a novel antibacterial delivery vehicle. The nisin-loaded nanoparticles were prepared using colloidal dispersion of the chitosan/alginate polymers in the presence of nisin. After the preparation of the nisin-loaded nanoparticles, their physicochemical properties such as size, shape, and zeta potential of the formulations were studied using scanning electron microscope and nanosizer instruments, consecutively. FTIR and differential scanning calorimetery studies were performed to investigate polymer-polymer or polymer-protein interactions. Next, the release kinetics and entrapment efficiency of the nisin-loaded nanoparticles were examined to assess the application potential of these formulations as a candidate vector. For measuring the antibacterial activity of the nisin-loaded nanoparticles, agar diffusion and MIC methods were employed. The samples under investigation for total microbial counts were pasteurized and raw milks each of which contained the nisin-loaded nanoparticles and inoculated Staphylococcus aureus (ATCC 19117 at 10(6) CFU/mL), pasteurized and raw milks each included free nisin and S. aureus (10(6) CF...Continue Reading

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Citations

Feb 18, 2014·Carbohydrate Polymers·Seyede Marzieh HosseiniRamin Khaksar
Oct 4, 2016·Frontiers in Microbiology·Hazem A FahimAhmed O El-Gendy
Apr 11, 2017·Critical Reviews in Food Science and Nutrition·Nathalie Almeida Lopes, Adriano Brandelli
Mar 1, 2018·Journal of Food Science and Technology·Eun Hee LeeDeog-Hwan Oh
Feb 15, 2020·Critical Reviews in Food Science and Nutrition·Sechul ChunJudy Gopal
Mar 25, 2017·Nanomaterials·Marta Álvarez-PainoMarta Fernández-García
Jul 14, 2020·Veterinary Research Communications·Laureano SchofsSergio F Sánchez Bruni
Feb 11, 2019·Biotechnology Letters·Gobinath ChandrakasanNorberto Chavarría-Hernández
Dec 29, 2020·Journal of Applied Microbiology·R Sulthana, A C Archer
Oct 30, 2020·Pharmaceuticals·Hazem ChoukaifeMulham Alfatama
Feb 6, 2021·Pharmaceutics·Gratiela Gradisteanu PircalabioruMariana-Carmen Chifiriuc
Oct 16, 2021·Journal of Agricultural and Food Chemistry·Shuhui ZhangAimin Ma

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