A comparative study of carotid atherosclerotic plaque microvessel density and angiogenic growth factor expression in symptomatic versus asymptomatic patients

European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
M ChowdhuryF Serracino-Inglott

Abstract

A challenge facing clinicians is identifying patients with asymptomatic carotid disease at risk of plaque instability. We hypothesise that locally released angiogenic growth factors contribute to plaque instability. Carotid endarterectomy specimens from eight symptomatic and eight asymptomatic patients were interrogated for microvessel density and angiogenic growth factor expression histologically using immunofluorescence, and biochemically using quantitative real-time polymerase chain reaction (q-RT-PCR). Bio-Plex suspension array was used to assess circulating biomarkers in venous blood from the same patients and six healthy age-matched controls. Immunofluorescence demonstrated significantly greater neovessel density in symptomatic plaques (P=0.010) with elevated expression of hepatocyte growth factor (HGF) (P=0.001) and its receptor MET (P=0.011) than in asymptomatic plaques. The q-RT-PCR demonstrated up-regulation of Endoglin (CD105), HGF (P=0.001) and MET (P=0.011) in the plaques of symptomatic versus asymptomatic patients. Bio-Plex suspension array demonstrated elevated HGF (P=0.002) serum levels in symptomatic versus asymptomatic patients and healthy controls, and decreased platelet-derived growth factor (PDGF) (P=0.036)...Continue Reading

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Citations

Nov 28, 2013·Cardiovascular Drugs and Therapy·John A Loudon
Dec 15, 2015·Archives of Clinical Neuropsychology : the Official Journal of the National Academy of Neuropsychologists·Daren C JacksonRobert J Dempsey
Dec 18, 2015·Clinical and Experimental Nephrology·Carmelo LibettaAntonio Dal Canton
Sep 10, 2014·International Journal of Stroke : Official Journal of the International Stroke Society·Kaavya NarasimhaluChristopher Chen
Sep 19, 2015·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Xin ShiPeng Qu

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