A comparative study of ethanol, hypoglycemia, hypoxia and neurotrophic factor interactions with fetal rat hippocampal neurons: a multi-factor in vitro model developmental ethanol effects

Brain Research. Developmental Brain Research
J J MitchellM B Heaton

Abstract

Fetal alcohol syndrome (FAS) is characterized by numerous central nervous system anomalies, with the hippocampus being particularly vulnerable to developmental ethanol exposure. In addition to direct ethanol neurotoxicity, other conditions resulting from maternal ethanol consumption, such as hypoglycemia and hypoxia, may also contribute to FAS. The present study used a tissue culture system to model multiple conditions which may relate to in vivo FAS, and assessed their relative neurotoxicity with MTT assays. Gestational day 18 rat hippocampal cultures were exposed to varying ethanol concentrations, glucose withdrawal-induced hypoglycemic (gwHG, 16 h) or acute hypoxic (aHP, 2 h) conditions alone, as well as to co-treatments with ethanol and gwHG or aHP. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have previously been shown to ameliorate ethanol-, hypoglycemia- and hypoxia-induced neurotoxicity. Therefore, their neuroprotective potential, along with ciliary neurotrophic factor (CNTF), was examined. Neuronal viability was reduced dose-dependently by ethanol, alone or with hypoglycemia or hypoxia. Ethanol + gwHG or aHP was not uniformly additive. NGF treatment provided the most extensive neuroprotection,...Continue Reading

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Citations

Mar 6, 2004·Neurotoxicology and Teratology·Jennifer D ThomasTeresa M O'Neill
Sep 6, 2000·Pharmacology, Biochemistry, and Behavior·Y WangJ C Lin
Apr 25, 2000·Brain Research. Developmental Brain Research·P A Yanni, T A Lindsley
May 24, 2003·Brain Research. Developmental Brain Research·Barbara WebbMarieta B Heaton
Feb 12, 1999·Brain Research. Developmental Brain Research·D M BradleyM B Heaton
Sep 3, 2011·Clinical and Experimental Pharmacology & Physiology·Stephen P GrayKaren M Moritz
May 8, 2000·Alcoholism, Clinical and Experimental Research·Z F CheemaR C Miranda
Jun 30, 2009·Seminars in Cell & Developmental Biology·Chris DeraufBarry Kosofsky
Nov 11, 2008·Neurobiology of Disease·Laurène Cagnon, Olivier Braissant
Oct 12, 2005·Brain Research Bulletin·Dawn C BarclayLaura M Mudd
Apr 4, 2016·Alcoholism, Clinical and Experimental Research·Elisabetta GeraceDomenico E Pellegrini-Giampietro
Aug 1, 2014·Frontiers in Genetics·Dan GoldowitzKristin M Hamre
Oct 18, 2007·Neurochemical Research·Li ZhuZhong Ming Qian
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shun-Kuo SunSu-Der Chen

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