A Comparative Study of Iron Uptake Rates and Mechanisms amongst Marine and Fresh Water Cyanobacteria: Prevalence of Reductive Iron Uptake

Life
Hagar LisYeala Shaked

Abstract

In this contribution, we address the question of iron bioavailability to cyanobacteria by measuring Fe uptake rates and probing for a reductive uptake pathway in diverse cyanobacterial species. We examined three Fe-substrates: dissolved inorganic iron (Fe') and the Fe-siderophores Ferrioxamine B (FOB) and FeAerobactin (FeAB). In order to compare across substrates and strains, we extracted uptake rate constants (kin = uptake rate/[Fe-substrate]). Fe' was the most bioavailable Fe form to cyanobacteria, with kin values higher than those of other substrates. When accounting for surface area (SA), all strains acquired Fe' at similar rates, as their kin/SA were similar. We also observed homogeneity in the uptake of FOB among strains, but with 10,000 times lower kin/SA values than Fe'. Uniformity in kin/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB. In contrast, different uptake pathways were found for FeAB along with variations in kin/SA. Our data supports the existence of a common reductive Fe uptake pathway amongst cyanobacteria, functioning alone or in addition to siderophore-mediated uptake. Cyanobacteria combining both uptake strategie...Continue Reading

References

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Citations

Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Katherine R M MackeyMak A Saito
Sep 3, 2016·Frontiers in Plant Science·Hanan SchoffmanNir Keren
Mar 11, 2018·Scientific Reports·Elizabeth D SwannerAndreas Kappler
Jul 30, 2019·Frontiers in Microbiology·Meri EichnerYeala Shaked
Sep 26, 2019·The ISME Journal·Nivi KesslerYeala Shaked
Jun 22, 2016·Plant Molecular Biology·Mareike RudolfEnrico Schleiff
May 15, 2019·Marine Drugs·Erland Årstøl, Martin F Hohmann-Marriott
Jul 28, 2020·Frontiers in Microbiology·Miyuki A ThirumurthyAnne K Jones
Sep 14, 2017·Current Microbiology·Michael M BabykinVladislav V Zinchenko
Aug 5, 2018·Applied and Environmental Microbiology·Guo-Wei QiuBao-Sheng Qiu
Nov 17, 2020·Environmental Microbiology·Guo-Wei QiuBao-Sheng Qiu
Feb 3, 2021·Nature Reviews. Microbiology·Andreas KapplerElizabeth D Swanner
Dec 3, 2020·Microorganisms·Annie Vera HunnestadMartin Frank Hohmann-Marriott
Dec 1, 2020·Frontiers in Microbiology·Robert SutakEmmanuel Lesuisse
Apr 6, 2021·Environmental Microbiology·Ling-Mei LiuHai-Bo Jiang
Apr 22, 2021·Journal of Microbiology and Biotechnology·Hannah SchätzleEnrico Schleiff
Jun 28, 2021·Trends in Microbiology·Guo-Wei QiuNir Keren
Jul 14, 2021·Environmental Science & Technology·Abeera A AnsariChul Park

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