A Comparative Study of Phosphatidylcholine versus Phosphatidylserine-Based Solid Supported Membranes for the Preparation of Liposome-Rich Interfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Alessio SacconiGiancarlo Margheri

Abstract

Solid supported membranes (SSMs) are usually formed by an hybrid octadecanethiol/phosphatidylcholine (PC) bilayer supported by a gold electrode. Recently, it was shown that phosphatidylserine (PS) in place of PC can promote a more effective accumulation of lipid vesicles on the SSM surface when Ca2+ and Mg2+ ions are present in the external environment. Here we performed a detailed comparative study of the vesicle adsorption process onto PC- and PS-SSMs by employing surface plasmon resonance (SPR), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM). SPR analysis has demonstrated a higher affinity of the PS-SSM surface for the phospholipid vesicles. Both SPR and EIS measurements suggest that adsorption of lipid vesicles on the PC-SSM tends to a saturating value, whereas a continuous and progressive vesicle adsorption occurs on the PS-SSM surface following subsequent liposome additions. AFM analysis pointed out a systematic flattening of the adsorbed vesicles on the PS-SSM surface. We interpreted our results as due to the strong coordinating action of the high amount of divalent cations accumulated at the negatively charged PS-SSM surface, whereas a lower amount of cations is present on the dipolar PC...Continue Reading

References

Sep 16, 2000·Physical Review Letters·C A KellerB Kasemo
Dec 6, 2002·Journal of the American Chemical Society·Vitalii I SilinAnne L Plant
Oct 29, 2003·Biophysical Journal·Ralf RichterAlain Brisson
Dec 6, 2005·Biophysical Journal·Michael PatraMikko Karttunen
Mar 22, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·E ReimhultB Kasemo
May 17, 2007·The Review of Scientific Instruments·I HorcasA M Baro
Mar 11, 2008·Archives of Biochemistry and Biophysics·Francesco Tadini-BuoninsegniKlaus Fendler
Aug 5, 2008·Methods : a Companion to Methods in Enzymology·Patrick SchulzKlaus Fendler
Feb 1, 1993·Biophysical Journal·E L Florin, H E Gaub
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·B P Oberts, G J Blanchard
May 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·B P Oberts, G J Blanchard
Jun 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Magdalena BrzozowskaPawel Krysinski
Jul 4, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·B P Oberts, G J Blanchard
Mar 5, 2013·Annual Review of Biophysics·Christof GrewerKlaus Fendler
Oct 18, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Alessio SacconiFrancesco Tadini-Buoninsegni
May 21, 2014·Analytical and Bioanalytical Chemistry·Daniel F KienleTonya L Kuhl
Apr 22, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Chao LiWei Zhan
Oct 17, 2017·Biochimica Et Biophysica Acta. Biomembranes·Tzong-Hsien LeeMarie-Isabel Aguilar
Nov 15, 2006·Surface Science Reports·Edward T Castellana, Paul S Cremer

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Citations

Jun 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Meryem BelkilaniCarole Chaix
Jul 24, 2021·ChemMedChem·Giacomo SordiFrancesco Tadini-Buoninsegni

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