PMID: 11335003May 4, 2001Paper

A comparative study of Purkinje cells in two RORalpha gene mutant mice: staggerer and RORalpha(-/-)

Brain Research. Developmental Brain Research
Mohamed DoulazmiJ Mariani

Abstract

The staggerer (Rora(sg/sg)) mutation is a deletion in the RORalpha gene, one member of a family of nuclear receptor genes related to the retinoic acid receptor. Recently Steinmayr et al. (Proc. Natl. Acad. Sci. USA 95 (1998) 3960) generated a RORalpha null-mutant mouse (Rora(-/-)) by using a targeting vector in which a beta-Gal gene replaces the second finger of the DNA-binding domain of RORalpha. The Rora(-/-) cerebellum is qualitatively a phenocopy of the Rora(sg/sg) one, but the two strains differ slightly in their motor skills. To address the question whether the morphological defects in the Rora(-/-) cerebellum are identical to the Rora(sg/sg) one, we compared number and size of Purkinje cells in both staggerer and RORalpha null-mutant mice, using calbindin (CaBP) immunohistochemistry and revelation of beta-Gal activity. Compared to control cerebella the Rora(sg/sg) cerebellum has 82% fewer CaBP-positive cells. In Rora(-/-) mouse, all the the beta-Gal-positive Purkinje cells also expressed CaBP, but the cerebellum contained 78% less CaBP-positive cells than control, a deficit not different from the one observed in Rora(sg/sg). We show similar mediolateral compartments in Purkinje cell number and cytological abnormality in ...Continue Reading

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Citations

Jul 29, 2010·Neural Development·Fatiha BoukhtoucheJean Mariani
Nov 26, 2008·The Journal of Comparative Neurology·Sonja JanmaatJohannes van der Want
Jan 1, 2015·Journal of Experimental Neuroscience·Ana Maria OlivaresNeena B Haider
Sep 17, 2004·Journal of Neurogenetics·Hadi ZanjaniJean Mariani
Jan 31, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Fatiha BoukhtoucheAlain Tedgui
Jun 30, 2006·Journal of Neurogenetics·Mohamed DoulazmiJean Mariani
Feb 14, 2014·Reviews in the Neurosciences·Alexey V ShevelkinMikhail V Pletnikov
Mar 2, 2019·The Cerebellum·Robert Lalonde, Catherine Strazielle
Mar 17, 2006·Journal of Neurogenetics·Nathalie JourniacBeatrice Vernet-der Garabedian

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