A Comparative Study of Receptor-Targeted Magnetosome and HSA-Coated Iron Oxide Nanoparticles as MRI Contrast-Enhancing Agent in Animal Cancer Model

Applied Biochemistry and Biotechnology
Ebru ErdalEmir B Denkbas

Abstract

Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bacterial magnetosome and HSA-coated iron oxide nanoparticles for targeting breast cancer. Both magnetosomes and HSA-coated iron oxide nanoparticles were chemically conjugated to fluorescent-labeled anti-EGFR antibodies. Antibody-conjugated nanoparticles were able to bind the MDA-MB-231 cell line, as assessed by flow cytometry. To compare the cytotoxic effect of nanoparticles, MTT assay was used, and according to the results, HSA-coated iron oxide nanoparticles were less cytotoxic to breast cancer cells than magnetosomes. Magnetosomes were bound with higher rate to breast cancer cells than HSA-coated iron oxide nanoparticles. While 250 μg/ml of magnetosomes was bound 92 ± 0.2%, 250 μg/ml of HSA-coated iron oxide nanoparticles was bound with a rate of 65 ± 5%. In vivo efficiencies of these nanoparticles on breast cancer generated in nude mice were assessed by MRI imaging. Anti-EGFR-modified nanoparticles provide higher...Continue Reading

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Citations

Dec 24, 2019·Nanomedicine·Faith Howard, Munitta Muthana
Aug 10, 2019·Applied Biochemistry and Biotechnology·Somayeh Akbari-KaradehSaeid Ghorbanzadeh-Mashkani
Oct 4, 2020·IET Nanobiotechnology·Şükran AlpdemirEmir Baki Denkbaş
Mar 25, 2019·Applied Microbiology and Biotechnology·Anissa DieudonnéSandra Prévéral
Oct 1, 2019·Current Pharmaceutical Design·Alessandra IscaroMunitta Muthana
May 8, 2021·Frontiers in Chemistry·Kumar Sachin, Santosh Kumar Karn
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bianca Elena-Beatrice CrețuVera Balan
Aug 29, 2021·Pharmaceutics·Slavko Kralj, Silvia Marchesan

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