A comparative study of serum total thyroxine estimation on unextracted serum by radioimmunoassay and by competitive protein binding.

Journal of Clinical Pathology
P MarsdenP J Howorth

Abstract

A rapid and precise radioimmunoassay (RIA) for serum total thyroxine (T4) on as little as 1-10 mul of unextracted serum is described. Results in hypothyroidism (overt and borderline), in euthyroid subjects in pregnant and oestrogen-medicated subjects, and in hyperthyroidism (overt and borderline) are compared with the results on the same sera by an established competitive protein binding technique (Ames' Tetralute) on unextracted serum from a different laboratory. The correlation between the two methods was excellent (r= 0.94) and no significant difference between overall appeared to measure total T4 Reliaby in sera containing only 1-3 or 2-6 nmol/1. Both methods predicted the clinical outcome in borderline hypothyroidism and borderline hyperthyroidism equally well and both gave normal results in T3-toxicosis. It is concluded that both techniques reliably measure total T4- RIA appears to have advantages of sensitivity and precision (especially in the hypothroid range), of simplicity, and of low cost.

References

Mar 1, 1975·Clinical Endocrinology·P Marsden, C G McKerron
Apr 1, 1971·The Journal of Clinical Endocrinology and Metabolism·L E BravermanS H Ingbar
Jun 1, 1972·The Journal of Clinical Endocrinology and Metabolism·I J Chopra
Oct 1, 1974·Clinical Endocrinology·W A RatcliffeT W Randall
Mar 24, 1972·Biochemical and Biophysical Research Communications·T MitsumaC S Hollander
May 30, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·O H GydeP J Howorth
Sep 1, 1971·The Journal of Clinical Endocrinology and Metabolism·H GharibT Hockert

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Citations

Mar 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·D S IthakissiosJ H Wicks
Jan 1, 1979·Journal of Immunological Methods·H R SchroederP O Vogelhut
Jul 1, 1975·British Journal of Obstetrics and Gynaecology·E O Akande, T D Hockaday
Apr 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse, O Lind
Nov 26, 2020·Mass Spectrometry Reviews·Marco BorsòAlessandro Saba
Sep 7, 1976·Biochemical and Biophysical Research Communications·S P BideyC G McKerron

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