A comparative study of stearic and lignoceric acid oxidation by human skin fibroblasts

Archives of Biochemistry and Biophysics
H Singh, A Poulos

Abstract

Sensitive assays were developed for long chain and very long chain fatty acid oxidation in human skin fibroblast homogenates. Stearic and lignoceric acids were degraded by the fibroblasts by the beta-oxidation pathway. The cofactor requirements for stearic and lignoceric acid beta-oxidation were very similar but not identical. For example, appreciable lignoceric acid oxidation could be demonstrated only in the presence of alpha-cyclodextrin and was inhibited by Triton X-100. In Zellweger's syndrome, stearic acid beta-oxidation was partially reduced whereas lignoceric acid beta-oxidation was reduced dramatically (less than 12% activity compared to the controls). The results presented suggest that stearic acid beta-oxidation occurs in mitochondria as well as in peroxisomes, but lignoceric acid oxidation occurs entirely in the peroxisomes. We suggest that the beta-oxidation systems for stearic acid and lignoceric acid may be different.

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Citations

Jan 1, 1990·Journal of Inherited Metabolic Disease·H SinghA Poulos
Sep 1, 1988·Lipids·E RuideraI Singh
Dec 16, 1988·Biochimica Et Biophysica Acta·I SinghT Hashimoto
Apr 2, 1990·Biochimica Et Biophysica Acta·U DiczfalusyI Björkhem
Dec 7, 1995·Biochimica Et Biophysica Acta·U DiczfalusyS E Alexson
Jun 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J WandersH van den Bosch
Jan 1, 1989·Progress in Lipid Research·A Poulos
Oct 2, 2001·Archives of Medical Research·R MenéndezS Jiménez
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O LazoI Singh
Jan 1, 1990·Journal of Inherited Metabolic Disease·R J WandersJ M Tager
Oct 11, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·R MenéndezJ L Alfonso
Feb 1, 1990·Biochemical Medicine and Metabolic Biology·Y Yoshida, I Singh
Nov 1, 1988·Archives of Biochemistry and Biophysics·H Singh, A Poulos
Aug 15, 1991·Biochemical and Biophysical Research Communications·B S Jakobs, R J Wanders
Dec 1, 1987·Archives of Biochemistry and Biophysics·H SinghA Poulos

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