A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices

Tissue Engineering and Regenerative Medicine
Yi ZhongHongcan Shi

Abstract

Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits. The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin-eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation. The biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them (p < 0.05). The histological staining results revealed that most of the mucosal epithelial cells were removed and that the decellularized...Continue Reading

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Citations

May 26, 2020·The International Journal of Artificial Organs·Zhihao WangHongcan Shi
Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Weston L NiermeyerTendy Chiang
Jul 4, 2020·Tissue Engineering and Regenerative Medicine·Hee-Woong YunByoung-Hyun Min
Jan 16, 2021·International Journal of Molecular Sciences·Óscar Darío García-GarcíaVíctor Carriel
Mar 20, 2021·Journal of Tissue Engineering·Mahsa Nouri BarkestaniSara Shamdani
Jan 14, 2022·Tissue Engineering and Regenerative Medicine·Mijin KimKyung-Sun Kang

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