PMID: 3754327Mar 25, 1986Paper

A comparative study of the regulation of cytochrome P-450 and glutathione transferase gene expression in rat liver

Nucleic Acids Research
V N FrancisG Padmanaban

Abstract

A cDNA clone for the Ya subunit of glutathione transferase from rat liver was constructed in E. coli. The clone hybridized to Ya and Yc subunit messenger RNAs. On the basis of experiments involving cell-free translation and hybridization to the cloned probe, it was shown that prototype inducers of cytochrome P-450 such as phenobarbitone and 3-methylcholanthrene as well as inhibitors such as CoCl2 and 3-amino-1,2,4-triazole enhanced the glutathione transferase (Ya+Yc) messenger RNA contents in rat liver. A comparative study with the induction of cytochrome P-450 (b+e) by phenobarbitone revealed that the drug manifested a striking increase in the nuclear pre-messenger RNAs for the cytochrome at 12 hr, but did not significantly affect the same in the case of glutathione transferase (Ya+Yc). 3-Amino-1,2,4-triazole and CoCl2 blocked the phenobarbitone mediated increase in cytochrome P-450 (b+e) nuclear pre-messenger RNAs. These compounds did not significantly affect the glutathione transferase (Ya+Yc) nuclear pre-messenger RNA levels. The polysomal, poly (A)- containing messenger RNAs for cytochrome P-450 (b+e) increased by 12-15 fold after phenobarbitone administration, reached a maximum around 16 hr and then decreased sharply. In ...Continue Reading

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Citations

Jan 2, 1989·European Journal of Biochemistry·G SrivastavaB K May
Aug 1, 1988·Archives of Biochemistry and Biophysics·G J Bhat, G Padmanaban
Mar 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T D Boyer
Dec 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T FournierD Porquet

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