A comparative study of tissue factor and kaolin on blood coagulation assays using rotational thromboelastometry and thromboelastography

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
Henry T PengPang N Shek

Abstract

Rotational thromboelastometry (ROTEM) and thromboelastography (TEG) have been increasingly used to diagnose acute coagulopathy and guide blood transfusion. The tests are routinely performed using different triggering activators such as tissue factor and kaolin, which activate different pathways yielding different results. To optimize the global blood coagulation assays using ROTEM and TEG, we conducted a comparative study on the activation methods employing tissue factor and kaolin at different concentrations as well as standard reagents as recommended by the manufacturer of each device. Key parameter values were obtained at various assay conditions to evaluate and compare coagulation and fibrinolysis profiles of citrated whole blood collected from healthy volunteers. It was found that tissue factor reduced ROTEM clotting time and TEG R, and increased ROTEM clot formation time and TEG K in a concentration-dependent manner. In addition, tissue factor affected ROTEM alpha angle, and maximum clot firmness, especially in the absence of kaolin activation, whereas both ROTEM and TEG clot lysis (LI30, CL30, and LY30) remained unaffected. Moreover, kaolin reduced ROTEM clotting time and TEG R and K, but to a lesser extent than tissue f...Continue Reading

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Citations

Oct 28, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·D E SchmidtA Ågren
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Software Mentioned

ROTEM
FIBTEM
INTEM
INTEM MCF
EXTEM
SPSS Statistics
INTEM CT

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