A comparative study on nonviral genetic modifications in cord blood and bone marrow mesenchymal stem cells.

Cytotechnology
Behnaz BakhshandehNasser Ghaemi

Abstract

The focus of both clinical and basic studies on stem cells is increasing due to their potentials in regenerative medicine and cell-based therapies. Recently stem cells have been genetically modified to enhance an existing character in or to bring a new property to them. However, accomplishment of declared goals requires detailed knowledge about their molecular characteristics which could be achieved by genetic modifications mostly through nonviral transfection strategies. Capable of differentiating into multiple cells, human unrestricted somatic stem cells (hUSSCs) and human mesenchymal stem cells (hMSCs) seem to be suitable candidates for transfection approaches. Involvement of microRNAs (miRNAs) in many biological processes makes their transfection evaluation valuable. Herein we investigated the efficacy and toxicity of four typically used transfection reagents (Arrest-In, Lipofectamine 2000, Oligofectamine and HiPerfect) systematically to deliver fluorescent labeled-miRNA and Green Fluorescent Protein (GFP) expressing plasmid into hUSSCs and hMSCs. The authenticity of stem cells was verified by differentiation experiments along with flow cytometry of surface markers. Our study revealed that stemness properties of these stem ...Continue Reading

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Citations

May 2, 2012·Biotechnology Letters·Behnaz BakhshandehMasoud Soleimani
Oct 12, 2012·In Vitro Cellular & Developmental Biology. Animal·Maryam HafiziAmir Atashi
Sep 4, 2012·Journal of Genetics·Behnaz BakhshandehNasser Ghaemi
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Mar 23, 2017·Cytotechnology·Zohreh JahanafroozBehnaz Bakhshandeh
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