PMID: 16532835Mar 15, 2006Paper

A comparative study on seeding methods of human mesenchymal stem cells in bone tissue engineering

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Xin QiXin-Xiang Xu

Abstract

Seeding is the crucial step in bone tissue engineering. In current study, static and dynamic seeding methods for human bone marrow stem cells (hBMSCs) were compared. The methods for assay of DNA content in the constructs after seeding were adopted. The optic microscopy for histological apearance and the scanning electron microscopy (SEM) and fluorescent RT-PCR for osteogenic markers were performed. The maximal initial seeding concentration in static seeding is lower than that in dynamic seeding. Histology and SEM revealed the even distribution and spreading of cells in the dynamically seeded constructs, but showed cell aggregation in the statically seeded counterparts. Fluorescent RT-PCR again revealed stronger osteogenic potential of dynamically seeded constructs. Therefore, this initial study demonstrated that dynamic seeding of human bone marrow stem cells is a promising technique in bone tissue engineering.

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