PMID: 2119591Aug 1, 1990Paper

A comparative study on the activation of J-774 macrophage-like cells by gamma-interferon, 1,25-dihydroxyvitamin D3 and lipopeptide RP-56142: ability to kill intracellularly multiplying Mycobacterium tuberculosis and Mycobacterium avium

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
N Rastogi, M C Blom-Potar

Abstract

The J-774 macrophage-like cell line has been established as a model for intracellular multiplication of pathogenic mycobacteria, permitting assessment of the intracellular bactericidal action of the macrophages after addition of both the drugs and immunomodulators. In this study, the action of immunomodulators was investigated. Significant morphological changes were demonstrated under the optical and scanning electron microscope (SEM), and the degree of macrophage activation was also measured by acid phosphatase (AcPase) cytochemistry, release of free oxygen radicals and by their ability to hinder the intracellular multiplication of virulent strains of Mycobacterium tuberculosis (M.tb) and Mycobacterium avium (M.av). For This purpose, the macrophages were left to multiply during 3 days in the presence of 50 U/ml of recombinant murine gamma-interferon (INF), 4 micrograms/ml of 1,25 dihydroxyvitamin D3 (D3) and 50 micrograms/ml of lipopeptide RP-56142 (RP) added separately or in various possible combinations, and these "activated" cells were then challenged with viable bacteria. Parallel controls included bacterial multiplication in nonactivated macrophages and also extracellularly but under the same experimental conditions as in...Continue Reading

References

Jun 10, 1988·European Journal of Pharmacology·J LefortB B Vargaftig
Jan 1, 1989·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·N RastogiH L David
Aug 30, 1985·Biochemical and Biophysical Research Communications·I Maridonneau-PariniB B Vargaftig
Feb 1, 1988·Antimicrobial Agents and Chemotherapy·N RastogiH L David
Aug 1, 1988·Biochimie·N Rastogi, H L David

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Citations

Aug 1, 1994·International Journal of Antimicrobial Agents·V St Georgiev
Jan 1, 1994·International Journal of Antimicrobial Agents·V S Georgiev
Nov 14, 2001·Infection and Immunity·S H El-EtrJ D Cirillo
Apr 2, 2013·Research in Veterinary Science·Shyamala ThirunavukkarasuRichard J Whittington
Aug 30, 2014·Endocrinology and Metabolism Clinics of North America·Gerome V EscotaWilliam G Powderly
Dec 20, 2002·Seminars in Pediatric Infectious Diseases·Paul F Riska, Stephen Carleton
May 20, 2015·Seminars in Cell & Developmental Biology·Muzlifah HaniffaMatthew Collin

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