A comparative study on the influence of an ivy preparation and an ivy/thyme combination on the β2 -adrenergic signal transduction

Heliyon
Hendrik BussmannHanns Häberlein

Abstract

The β2-adrenergic receptor (β2AR) is relevant for surfactant formation in alveolar type 2 cells and reduction of intracellular calcium concentration in bronchial muscle cells and thus for secretolytic and bronchospasmolytic effects. Herbal medicinal products that affect the β2AR system are used to treat common cold and bronchitis accompanied with mucus covered and narrowed airways. The present work compares the influence of an ivy preparation and an ivy/thyme combination on the β2-adrenergic signal transduction. For receptor binding studies and characterization of the lateral mobility of β2AR we have used single molecule detection by fluorescence correlation spectroscopy and single particle tracking. For the determination of both the second messenger cAMP and the internalization of β2AR we have generated luciferase based reporter cell lines, which produce a cAMP-dependent luciferase in the cytosol and express β2AR with extracellular luciferase moiety in the plasma membrane. While both preparations increased the β2AR binding, a significant increase of the cAMP level was observed only for the ivy preparation, which can be explained by the inhibited internalization of HiBiT-tagged β2AR under stimulating conditions. In contrast, is...Continue Reading

Citations

Jul 20, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Fabio MeurerSebastian Franken

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Methods Mentioned

BETA
fluorescence correlation spectroscopy
FCS
biosensor
transfection
PCR

Software Mentioned

SPT
Matlab Bayes analysis ( vbSPT )
Track
MATLAB
Fiji
MILLENIUM
vbSPT

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