A comparative ultrahistochemical study of glycosaminoglycans with cuprolinic blue in bone formed in vivo and in vitro

Calcified Tissue International
J R NefussiN Forest

Abstract

Histochemical and morphological studies have shown that proteoglygans (PG) are involved in mineralization process in vivo but such studies have not yet been conducted in vitro. A comparative histochemical study in electronic microscopy of the localization, organization, and morphology of the PG was performed with bones of calvaria rat formed in vivo and bone nodules formed in vitro from osteoblastic cells in culture. For this investigation, we used a cationic phthalocyanin dye, cuprolinic blue, in a critical electrolyte concentration which simultaneously stained the glycosaminoglycans and demineralized the bone. This histochemical technique demonstrated (1) osteoblast cells in vitro synthesized PG which were included in the matrix formed. (2) These PG were found in the calcified and uncalcified matrix both in vivo and in vitro. In the uncalcified matrix, PG were either free with a granular or rodlike structure or tightly connected to the periphery of the collagen fiber. Contrarily, in the calcified matrix, PG formed dense filamentous reticular patches between the collagen fibers. (3) Similarities in localization, organization, and morphology were noted in PG of bone formed de novo in vitro and in vivo with the exception of the ...Continue Reading

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Citations

Mar 1, 1992·Calcified Tissue International·J R NefussiM Goldberg
Nov 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J M SautierN Forest
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Apr 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R NefussiN Forest
Jan 1, 1996·Progress in Histochemistry and Cytochemistry·M Goldberg, A L Boskey

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