PMID: 9179590May 1, 1997Paper

A comparison between different methods for the determination of reduced and oxidized glutathione in mammalian tissues

Free Radical Research
M FloreaniP Palatini

Abstract

In this study, three rapid assay techniques for the determination of glutathione, one enzymatic, one fluorometric and one newly patented colorimetric method, were compared by measuring reduced (GSH) and oxidized (GSSG) glutathione in guinea-pig heart and liver. The HPLC technique was used as a standard, since it is considered the most reliable assay method. In heart, all methods measured the same levels of GSH (about 1 mumole/g wet tissue), whereas in liver the fluorometric assay gave GSH levels about half as high as those measured by the other methods (about 3 vs. 7 mumoles/g wet tissue). Conversely, the fluorometric assay grossly overestimated GSSG concentration (by 5 to 8 times) in both heart and liver. These results confirm previous doubts about the use of the fluorometric technique for GSSG determination in mammalian tissues and also raise some questions about its use for the measurement of GSH in liver. In this tissue, the GSH concentration determined by the fluorometric method was shown to be inversely correlated with the size of the sample, suggesting the presence of some quenching material.

References

Aug 1, 1977·Analytical Biochemistry·E Beutler, C West
Jan 1, 1993·Free Radical Research Communications·J M Gutteridge
Jun 1, 1993·Toxicology and Applied Pharmacology·S D AustR P Mason

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Citations

Feb 5, 2000·Molecular Reproduction and Development·J F BilodeauC Gagnon
Apr 14, 2011·In Vitro Cellular & Developmental Biology. Animal·Janka VaškováLadislav Vaško
Apr 23, 2013·In Vitro Cellular & Developmental Biology. Animal·Janka VaškováPál Perjési
Sep 12, 2003·Journal of Molecular and Cellular Cardiology·Shumin LiGeorge J Rozanski
Jul 11, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Maura FloreaniPietro Palatini
Oct 16, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Loriano BallarinArmando Sabbadin
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Emanuela Camera, Mauro Picardo
Dec 21, 2007·Antioxidants & Redox Signaling·Isabella Dalle-DonneRanieri Rossi
Jun 15, 2011·Journal of Medicinal Food·Min Hee KimMee Ree Kim
Sep 10, 2004·European Journal of Biochemistry·Lodovica VerganiFederica Dabbeni-Sala
Jun 1, 2014·The Journal of Microbiology·Chang-Jin LimKyunghoon Kim
Jun 24, 2011·Free Radical Research·Ahmed F El-ShafeyPeter G Arthur
Aug 9, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Muhammad Imran Khan, Zafar Iqbal
Feb 24, 2007·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Miroslav StỳbloMelinda A Beck
May 29, 2015·In Vitro Cellular & Developmental Biology. Animal·Vašková JankaMojžišová Gabriela
Mar 3, 2007·The Journal of Pharmacy and Pharmacology·Takako YokozawaYasuo Sei
Jan 28, 2006·Biochemical Pharmacology·Miguel StevensJan Balzarini
Jan 8, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·E J ChoK Y Park
Apr 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Martina ChripkovaJan Mojzis

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