A comparison between nucleus pulposus-derived stem cell transplantation and nucleus pulposus cell transplantation for the treatment of intervertebral disc degeneration in a rabbit model

International Journal of Surgery
Xiaofeng ChenZhicai Du

Abstract

In recent years, nucleus pulposus cell (NPC) transplantation has been used to treat intervertebral disc degeneration (IDD); however, the degenerative nature of NPCs influences its effectiveness. Nucleus pulposus-derived stem cells (NPSCs), which are self-renewing, have high expansion potential and can adapt to the intervertebral disc (IVD) microenvironment and may have a better regenerative capacity, which is favourable for treating IDD. The aim of this study was to compare the effectiveness of transplantation with NPSCs and NPCs on the regeneration of the IVD in rabbit models. NPSCs and NPCs were isolated from human degenerate nucleus pulposus tissue by differential adhesion method, and stem cell surface markers were detected by flow cytometry. Degenerative discs in rabbits were randomly distributed into three groups: NPSCs, NPCs and vehicle control group; the normal discs served as the normal control group. Cells of the P3 generation were prepared for transplantation. About 20 μl of cell suspension (NPSCs or NPCs) or DMEM was injected into corresponding discs, while the normal discs were left untreated. After 8 weeks, disc height was evaluated using X-ray, water content was evaluated by MRI, and gene and protein expression le...Continue Reading

References

Nov 21, 2002·Biochemical Society Transactions·J P G Urban
May 18, 2004·Arthritis and Rheumatism·Saifeddin AlsalamehMartin Lotz
Jan 18, 2005·The Spine Journal : Official Journal of the North American Spine Society·Seth C Gamradt, Jeffrey C Wang
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Thijs GrunhagenJill P G Urban
Jul 20, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Hans Joerg MeiselOlivera Alasevic
Aug 18, 2006·Spine·Michael A Adams, Peter J Roughley
Jan 19, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Akihiko HiyamaDaisuke Sakai
Oct 15, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Aiqun WeiAshish D Diwan
Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·C HohausH J Meisel
Feb 26, 2011·Journal of Pharmacology & Pharmacotherapeutics·Carol KilkennyDouglas G Altman
Sep 29, 2011·The Orthopedic Clinics of North America·Teija Lund, Thomas R Oxland
Oct 11, 2011·Lancet·Federico BalaguéChristine Cedraschi
Oct 29, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Bo HuangYue Zhou
May 23, 2012·Lancet·J H van DieënM A Adams
Feb 7, 2013·The Spine Journal : Official Journal of the North American Spine Society·Steven K LeckieJames D Kang
Dec 4, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Nevenka Kregar VelikonjaSally Roberts
Aug 15, 2014·American Journal of Physical Medicine & Rehabilitation·Shanmiao GouWenchun Qu

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Citations

Jul 13, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tomasz P LehmannPaweł P Jagodziński
Jul 28, 2019·International Journal of Molecular Sciences·Gauri TendulkarAndreas K Nüssler
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Yanbin ZhangLiming Xiong
Jun 19, 2017·Musculoskeletal Science & Practice·Ulrike H MitchellHaonan Wang

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