A comparison in normal individuals and sickle cell patients of reduced glutathione precursors and their transport between plasma and red cells

The Hematology Journal : the Official Journal of the European Haematology Association
K KiesslingJ C Ellory

Abstract

Reduced glutathione is an important antioxidant in red cells whose depletion may contribute to the pathophysiology of sickle cell disease. The current study was designed to examine the availability of reduced glutathione precursors (glutamate, cysteine, glycine and possibly glutamine) together with the activity of the main transport pathways for their uptake (system ASC for cysteine and glycine; system gly for glycine). Blood samples were obtained from normal (HbAA, HbA cells) and sickle cell disease patients (HbSS, HbS cells); amino acids were measured by HPLC; and transporter activity was measured by radioactive tracer fluxes (using serine and glycine for activity of system ASC; and glycine for that of system gly). Plasma concentrations of cysteine and glycine were increased and concentrations of all amino acids were elevated in HbS cells. The activity of system ASC was increased in HbS cells (both transport capacity and affinity were elevated for serine transport; transport capacity only for glycine). Activity of system gly was also increased (twofold increase in V(max) for glycine flux), though not significantly. Oxygenation also increased the activity of both transporters in normal and HbS cells. CO prevented deoxy-inhibit...Continue Reading

Citations

Jan 17, 2013·Revista brasileira de hematologia e hemoterapia·Lidiane de Souza TorresClaudia Regina Bonini-Domingos
Aug 16, 2016·Journal of Cardiovascular Pharmacology·Asya MakhroAnna Bogdanova
Dec 18, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olujide O OlubiyiBirgit Strodel
Jan 28, 2020·Experimental Biology and Medicine·Alina Sadaf, Charles T Quinn
Mar 7, 2020·British Journal of Haematology·Amina Nardo-MarinoDavid Rees
Jul 7, 2020·Frontiers in Physiology·Maggie L Kalev-ZylinskaAnna Bogdanova
Jan 26, 2006·American Journal of Physiology. Endocrinology and Metabolism·Marvin ReidFarook Jahoor
Jun 6, 2009·Redox Report : Communications in Free Radical Research·Stephney WhillierPhilip W Kuchel
Sep 18, 2010·European Journal of Haematology·Iryna RusanovaDario Acuña-Castroviejo
Dec 8, 2020·Platelets·Maggie L Kalev-ZylinskaAnna Y Bogdanova
Nov 12, 2018·Mayo Clinic Proceedings·Sargam KapoorLydia H Pecker
May 19, 2001·Blood Cells, Molecules & Diseases·J S Gibson
Oct 9, 2002·Blood Cells, Molecules & Diseases·J S Gibson, J C Ellory
Mar 25, 2004·Bioelectrochemistry·Clare DrewJohn S Gibson
Jun 5, 2007·The International Journal of Biochemistry & Cell Biology·Julia E RaftosPhilip W Kuchel
Sep 3, 2008·Biochemical and Biophysical Research Communications·Efemwonkiekie W IyamuGerald M Woods

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.