A comparison of an improved o-phthalaldehyde fluorometric method and high pressure liquid chromatography in the determination of brain 5-hydroxyindoles of rats treated with L-tryptophan and p-chlorophenyl-alanine

British Journal of Pharmacology
G CurzonM D Tricklebank

Abstract

1 The determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) by reaction with o-phthalaldehyde (OPT) in the presence of cysteine and subsequent fluorometry was compared with determination by a new high pressure liquid chromatography (h.p.l.c.) electrochemical method. 2 The methods were used to investigate a claim that the OPT method gives falsely high brain 5-HT values for rats given tryptophan (greater than 25 mg/kg i.p.) and as a consequence an inhibition of 5-HT synthesis by tryptophan is obscured. 3 High concentrations of tryptophan caused some increase of fluorescence when added to 5-HT solutions and carried through the OPT method, e.g. tryptophan (80 micrograms/ml) gave fluorescence identical to 5-HT (0.13 micrograms/ml). This interference was decreased by more than half and the sensitivity of the method increased if cysteine was added at more stages and fluorescence developed at 77 degrees C instead of 100 degrees C. 4 The h.p.l.c. and modified OPT methods did not give significantly different mean brain 5-HT or 5-HIAA values in rats given 0,25,100,250 mg/kg L-tryptophan though values were somewhat higher by the OPT method with the highest dose. This method gave significantly higher residual b...Continue Reading

References

Sep 1, 1975·Journal of Neurochemistry·G Curzon, C A Marsden
May 1, 1968·International Journal of Neuropharmacology·R P MaickelF P Miller

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Citations

Jul 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·E Mocaër-CretetC Jacquot
Nov 2, 2016·Hormone Molecular Biology and Clinical Investigation·Nirja K Chaudhari, Laxmipriya P Nampoothiri
Jan 1, 1986·Annals of the New York Academy of Sciences·P H HutsonG Curzon
Feb 1, 1986·Journal of Neurology, Neurosurgery, and Psychiatry·D NearyA N Davison
Apr 1, 1982·British Journal of Pharmacology·R Botting, A Morinan
Dec 1, 1984·Biochemical Pharmacology·M H FukamiK Ugurbil

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