A comparison of analytical methods for the determination of urea in yeast-based food and feed ingredients

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Louise FlannellyColm Anthony Moran

Abstract

Urea is a non-protein nitrogen compound, authorised in the EU as a nutritional source of nitrogen in animal feed intended for ruminants with a functional rumen. The Rapid Alert System for Food and Feed (RASFF) is the EU online platform through which food and feed safety risks are reported. During 2017, several rapid alerts were raised in the EU by individual member states regarding the presence of unlabelled urea in feed-grade yeast. The presence of urea in animal feed is analysed with a spectro-colorimetric method, which is the EU official control method. Other urea analytical methods include enzymatic and LC-MS methods, which are also investigated in this study. The main objectives of this study were to analyse yeast feed-grade and food-grade products with independent laboratories using the EU official control method, as well as enzymatic and LC-MS methods. Comparison of results from the spectro-colorimetric, enzymatic and LC-MS methods for reliability, consistency and accuracy determined the most reliable method for the quantification of urea in animal feed. Yeast food and feed samples were tested using four independent laboratories. Results demonstrated a lack of consistency both within the same laboratory and between separ...Continue Reading

References

Nov 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·C J Hallett, J G Cook
Mar 24, 2006·Nature·Nevan J KroganJack F Greenblatt
Mar 29, 2007·Animal Health Research Reviews·Khalid AbdounHolger Martens
May 4, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xinhua DaiRuifeng Xu

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