PMID: 6991329May 1, 1980Paper

A comparison of basal and insulin-stimulated glucose transport in rat adipocyte plasma membranes

Diabetes
C Ludvigsen, L Jarett

Abstract

Specific D-glucose transport in plasma membranes prepared from control and insulin-treated rat adipocytes was measured using a recently developed dual isotope ([3H]-D-glucose and [14C]-L-glucose), rapid filtration assay which allowed measurements of initial rates at 1 s and 37 degrees. Plasma membranes from insulin-treated adipocytes showed an increase in glucose transport compared with control cells. Saturation kinetic data revealed that the plasma membranes from insulin-treated and control cells had the same Km (26 mM) for glucose transport, whereas insulin treatment increased the Vmax from 4433 pmol/mg protein/s to 9465 pmol/mg proteins/s. Arrhenius plots showed no difference in the energy of activation between control and insulin-stimulated glucose transport states. The optimum pH of both control and insulin-stimulated glucose transport was 7.4. Lower or higher pHs progressively decreased both control and insulin-stimulated glucose transport proportionately. Calcium in the transport assay media did not affect basal or insulin-stimulated glucose transport. However, omission of calcium from the adipocyte incubation media significantly lowered the insulin stimulation by 24% while basal levels were not significantly affected. I...Continue Reading

Citations

Jul 25, 1985·Biochimica Et Biophysica Acta·D M MalchoffR G Langdon
Aug 20, 1981·Biochimica Et Biophysica Acta·W D Rees, G D Holman
Mar 1, 1982·Journal of Cellular Physiology·T P Ciaraldi, J M Olefsky
Jun 1, 1983·The American Journal of Physiology·I GottesmanJ Gerich

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