A Comparison of Bone Marrow and Cord Blood Mesenchymal Stem Cells for Cartilage Self-Assembly

Tissue Engineering. Part a
Jamie L WhiteKyriacos A Athanasiou

Abstract

Joint injury is a common cause of premature retirement for the human and equine athlete alike. Implantation of engineered cartilage offers the potential to increase the success rate of surgical intervention and hasten recovery times. Mesenchymal stem cells (MSCs) are a particularly attractive cell source for cartilage engineering. While bone marrow-derived MSCs (BM-MSCs) have been most extensively characterized for musculoskeletal tissue engineering, studies suggest that cord blood MSCs (CB-MSCs) may elicit a more robust chondrogenic phenotype. The objective of this study was to determine a superior equine MSC source for cartilage engineering. MSCs derived from bone marrow or cord blood were stimulated to undergo chondrogenesis through aggregate redifferentiation and used to generate cartilage through the self-assembling process. The resulting neocartilage produced from either BM-MSCs or CB-MSCs was compared by measuring mechanical, biochemical, and histological properties. We found that while BM constructs possessed higher tensile properties and collagen content, CB constructs had superior compressive properties comparable to that of native tissue and higher GAG content. Moreover, CB constructs had alkaline phosphatase activit...Continue Reading

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Citations

Dec 6, 2018·Journal of Biomechanical Engineering·Ryan P DonahueKyriacos Athanasiou
May 17, 2019·Stem Cells International·Barbara LukomskaKatarzyna Drela
Mar 17, 2021·Bulletin of Experimental Biology and Medicine·A V TsvetkovaK N Yarygin
Jul 13, 2021·World Journal of Stem Cells·Joice FülberRaquel Y A Baccarin
Oct 5, 2021·Tissue Engineering. Part B, Reviews·Sarah M Cooper, Roshni S Rainbow

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Methods Mentioned

BETA
flow cytometry
biopsy
Assay

Software Mentioned

ImageJ
FlowJo
MATLAB

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